Anti-Actin Regulatory Protein CAPG/MCP 抗体 [EPR13194-34] (ab181095)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13194-34] to Actin Regulatory Protein CAPG/MCP
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34]
Actin Regulatory Protein CAPG/MCP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13194-34] to Actin Regulatory Protein CAPG/MCP -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: THP-1, U937 and HeLa cell lysates.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR13194-34 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Positive Controls
-
Recombinant Protein
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181095の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (1) |
1/1000 - 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 38 kDa).
|
特記事項 |
---|
WB
1/1000 - 1/50000. Detects a band of approximately 39 kDa (predicted molecular weight: 38 kDa). |
ターゲット情報
-
機能
Calcium-sensitive protein which reversibly blocks the barbed ends of actin filaments but does not sever preformed actin filaments. May play an important role in macrophage function. May play a role in regulating cytoplasmic and/or nuclear structures through potential interactions with actin. May bind DNA. -
組織特異性
Macrophages and macrophage-like cells. -
配列類似性
Belongs to the villin/gelsolin family.
Contains 3 gelsolin-like repeats. -
翻訳後修飾
The N-terminus is blocked. -
細胞内局在
Cytoplasm. Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
参照データベース
- Entrez Gene: 822 Human
- Entrez Gene: 12332 Mouse
- Entrez Gene: 297339 Rat
- Omim: 153615 Human
- SwissProt: P40121 Human
- SwissProt: P24452 Mouse
- SwissProt: Q6AYC4 Rat
- Unigene: 516155 Human
see all -
別名
- Actin capping protein GCAP39 antibody
- Actin regulatory protein CAP G antibody
- Actin regulatory protein CAP-G antibody
see all
画像
-
All lanes : Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (ab181095) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CAPG knockout HeLa cell lysate
Lane 3 : THP-1 cell lysate
Lane 4 : U-937 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab181095 observed at 48 kDa. Red - loading control ab8245 observed at 36 kDa.
ab181095 Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] was shown to specifically react with Actin Regulatory Protein CAPG/MCP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265766 (knockout cell lysate ab257378) was used. Wild-type and Actin Regulatory Protein CAPG/MCP knockout samples were subjected to SDS-PAGE. ab181095 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (ab181095) at 1/10000 dilution + HeLa cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa -
Anti-Actin Regulatory Protein CAPG/MCP antibody [EPR13194-34] (ab181095) at 1/50000 dilution + U937 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab181095 は論文での使用が確認できていません。