Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
African Green Monkey Cell (COS7)
Specification
COS7
Permeabilization
Yes - Triton X 100 0.3%
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: cy3 jackson labs goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
Shows nuclear localization after permeabilization with triton or with methanol fixation (methanol fixation causes clumped staining). Antibody shows nuclear as well as cytoplasmic localization. Overall staining is diffuse. Image attached (red - 1801 ab, blue - DAPI). Does not detect microfilaments. Shows clean western blot band at ~40kDA of Cos7 WCE.
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投稿 Jun 03 2013