Anti-Actin 抗体 [AC-40] (ab11003)
Key features and details
- Mouse monoclonal [AC-40] to Actin
- Suitable for: ELISA, IHC-P, IHC-Fr, ICC/IF, WB, IHC-FoFr, Dot blot
- Reacts with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Carp, Snail
- Isotype: IgG2a
製品の概要
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製品名
Anti-Actin antibody [AC-40]
Actin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [AC-40] to Actin -
由来種
Mouse -
アプリケーション
適用あり: ELISA, IHC-P, IHC-Fr, ICC/IF, WB, IHC-FoFr, Dot blotmore details -
種交差性
交差種: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Hamster, Cow, Dog, Human, Pig, Xenopus laevis, Carp, Snail -
免疫原
Synthetic peptide within Human Actin aa 365-375 (C terminal). The exact sequence is proprietary.
Sequence:SGPSIVHRKCF
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エピトープ
Monoclonal anti-Actin recognizes an epitope located on the C-terminal end of actin. This epitope is conserved in all actin isoforms. -
特記事項
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Tissue culture supernatant -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
AC-40 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11003の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA | (1) |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
IHC-FoFr |
Use at an assay dependent concentration.
|
|
Dot blot |
Use at an assay dependent concentration.
|
特記事項 |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. 1/200 determined by indirect immunofluorescent staining of cultured Human or chicken fibroblasts. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa. 1/500 using cultured Human or chicken fibroblast extract. Predicted molecular weight: 42 kDa. |
IHC-FoFr
Use at an assay dependent concentration. |
Dot blot
Use at an assay dependent concentration. |
ターゲット情報
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機能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
関連疾患
Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. -
配列類似性
Belongs to the actin family. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 421534 Chicken
- Entrez Gene: 281592 Cow
- Entrez Gene: 58 Human
- Entrez Gene: 11459 Mouse
- Entrez Gene: 100154254 Pig
- Entrez Gene: 29437 Rat
- Omim: 102610 Human
- SwissProt: P68139 Chicken
see all -
別名
- a actin antibody
- a-actin antibody
- ACTA antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (49)
ab11003 は 49 報の論文で使用されています。
- Martins CS et al. Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells. J Cell Biol 222:N/A (2023). PubMed: 36562751
- Chong WC et al. Atypical Teratoid Rhabdoid Tumours Are Susceptible to Panobinostat-Mediated Differentiation Therapy. Cancers (Basel) 13:N/A (2021). PubMed: 34680294
- Yu Q et al. MDA5 disease variant M854K prevents ATP-dependent structural discrimination of viral and cellular RNA. Nat Commun 12:6668 (2021). PubMed: 34795277
- Jäckle A et al. Sitagliptin and the Blood-Retina Barrier: Effects on Retinal Endothelial Cells Manifested Only after Prolonged Exposure. J Diabetes Res 2020:2450781 (2020). PubMed: 32566677
- Pang N et al. Endothelial cell-derived CCL15 mediates the transmigration of fibrocytes through the CCL15-CCR1 axis in vitro. Mol Med Rep 22:5339-5347 (2020). PubMed: 33174007