製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Aconitase 2
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human, Caenorhabditis elegans
    交差が予測される動物種: Rat, Chicken, Cow, Dog, Pig, Saccharomyces cerevisiae, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 648-697 (

    RYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHET

    ) of human ACO2 (NP_001089).

  • ポジティブ・コントロール
    • 721_B cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

IHC-P 1/100.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 85 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pineal tissue labelling Aconitase 2 with ab83528 at 1/100. A Cy3-conjugated donkey anti-rabbit IgG (1/200) was used as the secondary antibody. Positive staining shown in the cytoplasm of the cell bodies of penealocytes and connective tissue (septum). Magnification: 20X. Exposure time: 0.5 - 2.0 seconds. Left - DAPI. Middle - Aconitase 2. Right - Merge.
  • Anti-Aconitase 2 antibody (ab83528) at 1 µg/ml (in 5% skim milk/ PBS) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 85 kDa
    Observed band size: 85 kDa

  • ICC/IF image of ab83528 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83528, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Fischer F  et al. RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model. Sci Rep 5:12697 (2015). Read more (PubMed: 26220011) »
  • Scheving R  et al. Protein S-nitrosylation and denitrosylation in the mouse spinal cord upon injury of the sciatic nerve. J Proteomics 75:3987-4004 (2012). WB, ELISA ; Mouse . Read more (PubMed: 22588120) »
See all 2 Publications for this product

レビューと Q&A

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Caenorhabditis elegans Cell lysate - whole cell (Whole worm homogenate)
Loading amount
80 µg
Specification
Whole worm homogenate
Gel Running Conditions
Reduced Denaturing (10 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Michal Bejerano-Sagie

Verified customer

投稿 Jan 17 2012

Answer

DISCOUNT CODE: 41541 Expiration date: 17/04/2012 I am very pleased to hear you would like to accept our offer and test ab83528 in C.elegans. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for C. elegans and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your enquiry and interest. The cross-reactivity of this antibody with C.elegans has not been tested, though it is predicted due to sequence homology. I could certainly offer a discount code for testing. Could you please pass the customer's name, e-mail address and the institute? Have a nice day! I look forward to hearing from you soon.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Tissue lysate - whole (whole flies)
Loading amount
15 µg
Specification
whole flies
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

投稿 Nov 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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