Acetylcholinesterase Assay Kit (Colorimetric) (ab138871)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell culture extracts, Cell Lysate, Plasma, Serum, Tissue Lysate
- Sensitivity: 1 mU/ml
製品の概要
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製品名
Acetylcholinesterase Assay Kit (Colorimetric)
Acetylcholinesterase キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Serum, Plasma, Cell culture extracts, Cell Lysate, Tissue Lysate -
アッセイタイプ
Enzyme activity -
検出感度
1 mU/ml -
全工程の試験時間
0h 30m -
製品の概要
Acetylcholinesterase Assay Kit ab138871 provides a convenient colorimetric assay for the detection of AChE activity in blood, cell extracts and other samples from mammals and other species.
The acetylcholinesterase assay protocol uses DTNB to quantify the thiocholine produced from the hydrolysis of acetylthiocholine by AChE. The absorption intensity of DTNB adduct (410 nm) is used to measure the amount of thiocholine formed, which is proportional to the AChE activity.
The assay will detect as little as 0.1 mU AChE in a 100 µL assay volume (1 mU/mL).
Acetylcholinesterase assay protocol summary:
- add standards and samples to wells
- add acetylthiocholine reaction mix and incubate for 10-30 min at room temp
- analyze with a microplate reader -
特記事項
Please note this product does not differentiate between acetylcholesterase (AchE) or butyrylcholinesterase (BChE) activity as both enzymes can hydrolyze acetylcholine. However, an acetylcholinesterase inhibitor like Donepezil hydrochloride can be used as a control.
If you are looking for a fluorometric kit, we recommend Acetylcholinesterase Assay Kit (Fluorometric-Green) (ab133872) (Ex/Em = 490/520 nm) or Acetylcholinesterase Assay Kit (Fluorometric-Red) (ab133873) (Ex/Em = 540/590 nm).
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 200 tests Acetylcholinesterase Standard 1 x 5 units Acetylthiocholine 1 vial Assay Buffer 1 x 25ml DTNB 1 vial -
研究分野
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機能
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis. -
組織特異性
Isoform H is highly expressed in erythrocytes. -
配列類似性
Belongs to the type-B carboxylesterase/lipase family. -
細胞内局在
Cell membrane; Cell junction > synapse. Secreted. Cell membrane and Nucleus. Only observed in apoptotic nuclei. - Information by UniProt
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別名
- ACEE
- ACES_HUMAN
- Acetylcholinesterase
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関連製品
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Related Products
画像
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ab138871 was used to determine AChE activity in the cortex and in the hippocampus. AChE activity was measured at 410 nm.
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AChE measured in mouse tissue lysates showing quantity (mU) per mg protein of tested sample
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AChE measured in cell lysates showing quantity (mU) per 1 mln of tested cells
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AChE measured in biological fluids showing quantity (mU) per mL of tested sample
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Acetylcholinesterase dose response was measured in a white/clear bottom 96-well plate with ab138871 Acetylcholinesterase Assay Kit (Colorimetric) using a microplate reader. As low as 0.1 mU/well of acetylcholinesterase can be detected with 30 minutes incubation (n=3)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (46)
ab138871 は 46 報の論文で使用されています。
- Zhang H et al. The Effect of Guilingji Capsules on Vascular Mild Cognitive Impairment: A Randomized, Double-Blind, Controlled Trial. Evid Based Complement Alternat Med 2022:4778163 (2022). PubMed: 35116067
- Aykac A et al. A multi-parameter evaluation of the neuroprotective and cognitive-enhancing effects of Origanum onites L. (Turkish Oregano) essential oil on scopolamine-induced amnestic rats. Metab Brain Dis 37:1041-1055 (2022). PubMed: 35201555
- Girard MP et al. Chemical Synthesis and Biological Activities of Amaryllidaceae Alkaloid Norbelladine Derivatives and Precursors. Molecules 27:N/A (2022). PubMed: 36080382
- Guo Q et al. Novel small molecular compound 2JY-OBZ4 alleviates AD pathology in cell models via regulating multiple targets. Aging (Albany NY) 14:8077-8094 (2022). PubMed: 36227154
- Uras G et al. Development of p-Tau Differentiated Cell Model of Alzheimer's Disease to Screen Novel Acetylcholinesterase Inhibitors. Int J Mol Sci 23:N/A (2022). PubMed: 36499118