製品名Anti-Acetylated Proteins antibody - ChIP Grade
製品の詳細Rabbit polyclonal to Acetylated Proteins - ChIP Grade
特異性The prime activity is against the tri and tetra acetylated forms of histone H4. It also reacts with epsilon acetylated lysines. It also reacts with other acetylated proteins: BSA, E2F, PCAF, Importin, GATA1, BRCA2 and Rb.
アプリケーション適用あり: WB, IP, ELISA, ChIPmore details
Chemically acetylated human histone H4
- mammalian histones
This antibody recognises a wide range of acetylated proteins.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
一次抗体 備考This antibody recognises a wide range of acetylated proteins.
ChIP Related Products
Our Abpromise guarantee covers the use of ab193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
|ChIP||Use at an assay dependent dilution. Recommended to use 5-10µl for 25µg of chromatin.|
Chromatin was prepared from Hela cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25µg of chromatin, 5µl of ab193 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.
ab193 used in Western Blotting.
Lysate prepared from T47D cells was loaded at 100µg. Cells were pre-treated with either insulin, butyrate, resveratrol or trichostatin A. Primary antibody incubated for 24 hours at a 1/1000 dilution. An HRP-conjugated F(ab)2 secondary antibody was used at a 1/8000 dilution.
Left arrows indicate signal increased by treatment with inhibitors of deacetylases. The nuclear protein increased by butyrate and trichostatin A is probably histone H4 based on its size. One unidentified protein of approximately 65kDa is increased by the treatments in cytoplasm.
This product has been referenced in:
- Li Y et al. Post-translational regulation of a Porphyromonas gingivalis regulator. J Oral Microbiol 10:1487743 (2018). Read more (PubMed: 29988788) »
- Ma S et al. SIRT1 Activation by Resveratrol Alleviates Cardiac Dysfunction via Mitochondrial Regulation in Diabetic Cardiomyopathy Mice. Oxid Med Cell Longev 2017:4602715 (2017). Read more (PubMed: 28883902) »