Anti-acetyl Lysine 抗体 [RM101] (ab190479)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [RM101] to acetyl Lysine
- Suitable for: ELISA, WB, IHC-P, ChIP, Flow Cyt, IP, ICC/IF
- Reacts with: Species independent
製品の概要
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製品名
Anti-acetyl Lysine antibody [RM101]
acetyl Lysine 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [RM101] to acetyl Lysine -
由来種
Rabbit -
特異性
ab190479 reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, or lysine with other modification. -
アプリケーション
適用あり: ELISA, WB, IHC-P, ChIP, Flow Cyt, IP, ICC/IFmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide corresponding to acetyl Lysine conjugated to bovine serum albumin.
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ポジティブ・コントロール
- A431 cells treated with Trichostatin A; HeLa whole cell lysate - Trichostatin A treated
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
RM101 -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab190479の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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WB | (1) |
1/500 - 1/2000.
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IHC-P |
1/100 - 1/500.
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ChIP |
1/100 - 1/500.
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Flow Cyt |
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP |
1/100 - 1/500.
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ICC/IF |
1/100 - 1/500.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. |
IHC-P
1/100 - 1/500. |
ChIP
1/100 - 1/500. |
Flow Cyt
Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/100 - 1/500. |
ICC/IF
1/100 - 1/500. |
ターゲット情報
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関連性
In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo. -
別名
- pan acetyl Lysine antibody
画像
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Lane 1: A431 whole cell lysate
Lane 2: A431 whole cell lysate (pretreated with Trichostatin A)
Lane 3: A431 whole cell lysate immunoprecipitated with Rabbit IgG
Lane 4: A431 whole cell lysate (pretreated with Trichostatin A) immunoprecipitated with Rabbit IgG
Lane 5: A431 whole cell lysate immunoprecipitated with ab190479 at 1/500
Lane 6: A431 whole cell lysate (pretreated with Trichostatin A) immunoprecipitated with ab190479 at 1/500Western blot performed using anti-PTEN mouse monoclonal antibody.
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Immunocytochemical staining of HeLa cells labelling Acetyl Lysine with ab190479 at 1:100. Actin filaments are labelled using fluorescein phalloidin (green), and nuclei are stained with DAPI (blue).
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All lanes : Anti-acetyl Lysine antibody [RM101] (ab190479) at 1/2000 dilution
Lanes 1 & 3 & 5 : Lysate of nontreated HeLa cells
Lanes 2 & 4 & 6 : Lysate of HeLa cells treated with Trichostatin A
Developed using the ECL technique.Exposure time increased from blot on left (lanes 1, 2) to blot on right (lanes 5,6).
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Immunofluorescent analysis of A431cells nontreated (left) or treated with Trichostatin A (right), using ab190479 at 1/500 followed by a PE conjugated secondary antibody (red) and DAPI (blue).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab190479 は 19 報の論文で使用されています。
- Lin JX et al. m6A methylation mediates LHPP acetylation as a tumour aerobic glycolysis suppressor to improve the prognosis of gastric cancer. Cell Death Dis 13:463 (2022). PubMed: 35568711
- Hao K et al. Nicotinamide reverses deficits in puberty-born neurons and cognitive function after maternal separation. J Neuroinflammation 19:232 (2022). PubMed: 36131290
- Tao Z et al. Sirt1 coordinates with ERa to regulate autophagy and adiposity. Cell Death Discov 7:53 (2021). PubMed: 33723227
- Wang ZH et al. Nicotinamide Riboside Enhances Endothelial Precursor Cell Function to Promote Refractory Wound Healing Through Mediating the Sirt1/AMPK Pathway. Front Pharmacol 12:671563 (2021). PubMed: 34054544
- Ni Y et al. Silent information regulator 2 promotes clear cell renal cell carcinoma progression through deacetylation and small ubiquitin-related modifier 1 modification of glucose 6-phosphate dehydrogenase. Cancer Sci 112:4075-4086 (2021). PubMed: 34310804