Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-acetyl Lysine 抗体 [1C6] (ab22550)

Reviews (5)Q&A (3)References (11)
Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [1C6] (ab22550)
  • ChIP - Anti-acetyl Lysine antibody [1C6] (ab22550)
  • Western blot - Anti-acetyl Lysine antibody [1C6] (ab22550)
  • Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [1C6] (ab22550)

Key features and details

  • Mouse monoclonal [1C6] to acetyl Lysine
  • Suitable for: ChIP, ICC/IF, WB
  • Reacts with: Species independent
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-acetyl Lysine antibody [1C6]
    acetyl Lysine 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [1C6] to acetyl Lysine

  • 由来種

    Mouse

  • 特異性

    ab22550 recognises proteins with acetylated lysine.

  • アプリケーション

    適用あり: ChIP, ICC/IF, WBmore details

  • 種交差性

    交差種: Species independent

  • 免疫原

    Synthetic peptide: sequence surrounding the acetylated lysine 9 of histone H3

  • ポジティブ･コントロール

    • ChIP: HeLa cell lysate. ICC: MCF7 cell line. WB: HeLa, COS7 and C2C12 cell lysates.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...

  • 精製度

    Protein G purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    1C6

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab22550の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ChIP (1)
Use a concentration of 10 µg/ml.
ICC/IF
1/100 - 1/500.
WB (3)
1/500 - 1/2000.
特記事項
ChIP
Use a concentration of 10 µg/ml.
ICC/IF
1/100 - 1/500.
WB
1/500 - 1/2000.

ターゲット情報

  • 関連性

    In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation. The reversible lysine acetylation of histones and non-histone proteins plays a vital role in the regulation of many cellular processes including chromatin dynamics and transcription, gene silencing, cell cycle progression, apoptosis, differentiation, DNA replication, DNA repair, nuclear import, and neuronal repression. More than 20 acetyltransferases and 18 deacetylases have been identified so far, but the mechanistic details of substrate selection and site specificity of these enzymes remain unclear. Over 40 transcription factors and 30 other nuclear, cytoplasmic, bacterial, and viral proteins have been shown to be acetylated in vivo.

  • 別名

    • pan acetyl Lysine antibody

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [1C6] (ab22550)
    Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [1C6] (ab22550)

    Immunoflouroescence analysis of HeLa Cells labelling lysine acetylated proteins with ab22550. Formalin fixed cells were permeabalized with 0.1& Triton X-100 in TBS for 10 mins at room temperature and subsequently blocked with BSA at room temperature for 15 mins. Cells were then probed with ab22550 at 1/100 for 1 hour at room temperature. The secondary used was a DyLight® 488 goat anti-mouse used at 1/400 for 30 minutes at room temperature. Additional counterstains used were F-actin with a DyLight® 554 Phalloidin and Neuclei stained using a Hoechst 33342 conjugate. Image was taken at X20 magnification.

  • ChIP - Anti-acetyl Lysine antibody [1C6] (ab22550)
    ChIP - Anti-acetyl Lysine antibody [1C6] (ab22550)

    Chromatin Co-Immunoprecipitation (ChIP) analysis  using ab22550 binding acetylated lysines in 10E+06 LNCaP cells. Protein binding was detected using real-time PCR.

    Positive control: Fold enrichment of ab22550.
    Negative Control: Non-specific IgG.

  • Western blot - Anti-acetyl Lysine antibody [1C6] (ab22550)
    Western blot - Anti-acetyl Lysine antibody [1C6] (ab22550)
    All lanes : Anti-acetyl Lysine antibody [1C6] (ab22550) at 1/1000 dilution

    Lane 1 : HeLa (untreated) cell lysate
    Lane 2 : HeLa (treated with 0.3 uM TSA, 16h) cell lysate
    Lanes 3 & 6 : HeLa (treated with 3 uM TSA, 16h) cell lysate
    Lane 4 : COS7 (untreated) cell lysate
    Lane 5 : COS7 (treated with 0.3 uM TSA, 16h) cell lysate
    Lane 7 : C2C12 (untreated) cell lysate
    Lane 9 : C2C12 (treated with 3 uM TSA, 16h) cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Lane 8 : C2C12 (treated with 0.3 uM TSA, 16h) cell lysate


    Western blot analysis of lysine acetylated proteins from cells left untreated (DMSO only) or cells treated with 0.3uM or 3uM of Trichostatin A (TSA) for 16 hours was performed by loading 50 µg of the indicated whole cell lysates per well and 10 µL of PageRuler Prestained Protein Ladder onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an Acetyl Lysine monoclonal antibody at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:20,000 for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [1C6] (ab22550)
    Immunocytochemistry/ Immunofluorescence - Anti-acetyl Lysine antibody [1C6] (ab22550)
    ICC/IF image of ab22550 stained MCF7 cells. The cells were 4% formaldehye fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22550, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献 (11)

ab22550 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab22550 は 11 報の論文で使用されています。

  • Lv T  et al. Immunoprecipitation of Acetyl-lysine And Western Blotting of Long-chain acyl-CoA Dehydrogenases and Beta-hydroxyacyl-CoA Dehydrogenase in Palmitic Acid Treated Human Renal Tubular Epithelial Cells. Bio Protoc 10:e3765 (2020). PubMed: 33659423
  • Hu Z  et al. Momordica charantia polysaccharides modulate the differentiation of neural stem cells via SIRT1/?-catenin axis in cerebral ischemia/reperfusion. Stem Cell Res Ther 11:485 (2020). PubMed: 33198798
  • Yi X  et al. SIRT3-Dependent Mitochondrial Dynamics Remodeling Contributes to Oxidative Stress-Induced Melanocyte Degeneration in Vitiligo. Theranostics 9:1614-1633 (2019). PubMed: 31037127
  • Farkas M & McMahon SB Rapid Detection of p53 Acetylation Status in Response to Cellular Stress Signaling. Methods Mol Biol 1983:255-262 (2019). PubMed: 31087303
  • Lin Q  et al. RELMa Licenses Macrophages for Damage-Associated Molecular Pattern Activation to Instigate Pulmonary Vascular Remodeling. J Immunol 203:2862-2871 (2019). PubMed: 31611261
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-8 of 8 Abreviews or Q&A

Western blot abreview for Anti-acetyl Lysine antibody [1C6]

 Good
Abreviews
Application
Western blot
Sample
Rat Cell lysate - other (liver)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
20 µg
Specification
liver
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

投稿 Sep 30 2021

ChIP abreview for Anti-acetyl Lysine antibody [1C6]

 Good
Abreviews
abreview image
Application
ChIP
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Negative control
mouse IgG
Specification
primary hepatoctyes
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Positive control
H2AZ
Read More

Abcam user community

Verified customer

投稿 Dec 15 2014

Immunoprecipitation abreview for Anti-acetyl Lysine antibody [1C6]

 Average
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Total protein in input
20 µg
Immuno-precipitation step
Protein A
Specification
primary hepatoctyes
Read More

Abcam user community

Verified customer

投稿 Dec 15 2014

Western blot abreview for Anti-acetyl Lysine antibody [1C6]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hepatocyte)
Gel Running Conditions
Non-reduced Denaturing (15)
Loading amount
30 µg
Specification
Hepatocyte
Blocking step
I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
Read More

Abcam user community

Verified customer

投稿 Mar 11 2014

Western blot abreview for Anti-acetyl Lysine antibody [1C6]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat cells)
Gel Running Conditions
Reduced Denaturing (10% SDS PAGE)
Loading amount
10 µg
Treatment
trichostatin A 250 nM treated cells for 24h
Specification
Jurkat cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 May 19 2010

Question

Is this antibody has ever been tested in Immunoprecipitation?

Read More
Answer

Thank you very much for your interest in ab22550. To our knowledge, ab22550 has not been tested in IP. Therefore, I can offer a discount off a future purchase if you buy ab22550 now, test it in IPand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab22550 in IP. I will then send a discount code. This code must be issued before purchasing ab22550 so please wait for my reply before ordering. 2. Purchase ab22550 either by phone, fax, or online (www.abcam.com). 3. Test it in IP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab22550 turns out to be unsuitable for IP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Question

I would like to do some experiments to detect the lysine-specific acetylation of a non-histone protein. There is no sequence homology between the histone acetylation site used as the antigen to develop the antibody. Will the monoclonal acetylation antibody work to detect acetyl-lysine residues on any peptide?

Read More
Answer

Thank you for your enquiry. The synthetic peptide used to raise this antibody was a peptide sequence surrounding the acetylated lysine 9 of histone H3. This antibody has been shown to generate a ladder in a HeLa whole cell lysate demonstrating that it has the potential to recognise acetylated, non-histone proteins. I cannot guarantee that it will recognise every acetyl-lysine epitope given that each epitope will vary dependent on the adjacent residues. May I suggest that you consider our monoclonal N-epsilon acetyl Lysine antibody [11A1] (ab409). Mike Schutkowski has left a favourable review following it application against various acetylated lysine peptides. Please do not hesitate to contact me should you require further assistance.

Read More

Question

Do you have any information aboutthe ability of this antibody to react with other non-histone acetylated proteins?

Read More
Answer

Thank you for your enquiry. Yes, by Western blot, this antibody detects a ladder of acetylated proteins in HeLa cell lysates. In immunofluorescence procedures, this antibody recognizes acetylated lysine in gamma irradiated HeLa cells. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com