リコンビナントRabMAb

Anti-Acetyl Coenzyme A carboxylase alpha 抗体 [EPR23235-47] - BSA and Azide free (ab272703)

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Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Flow Cytometry - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
  • Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23235-47] to Acetyl Coenzyme A carboxylase alpha - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free
    Acetyl Coenzyme A carboxylase alpha 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23235-47] to Acetyl Coenzyme A carboxylase alpha - BSA and Azide free

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
    適用なし: IHC-Fr

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: 293T, HeLa, PC-12 and C2C12 lysates. IHC-P: Human breast carcinoma and endometrial carcinoma tissue. Mouse and rat lung tissue. ICC/IF: C2C12 and 293T cells. Flow Cyt: C2C12 and 293T cells. IP: C2C12 and 293T cells.

  • 特記事項

    ab272703 is the carrier-free version of ab269272. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

アプリケーション

Our Abpromise guarantee covers the use of ab272703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 265 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for IHC-Fr.

    • ターゲット情報

    • 機能

      Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase.

    • 組織特異性

      Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver.

    • パスウェイ

      Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1.

    • 関連疾患

      Defects in ACACA are a cause of acetyl-CoA carboxylase 1 deficiency (ACACAD) [MIM:200350]; also known as ACAC deficiency or ACC deficiency. An inborn error of de novo fatty acid synthesis associated with severe brain damage, persistent myopathy and poor growth.

    • 配列類似性

      Contains 1 ATP-grasp domain.
      Contains 1 biotin carboxylation domain.
      Contains 1 biotinyl-binding domain.
      Contains 1 carboxyltransferase domain.

    • 翻訳後修飾

      Phosphorylation on Ser-1263 is required for interaction with BRCA1.

    • 細胞内局在

      Cytoplasm.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • ACAC antibody
      • ACACA antibody
      • ACACA_HUMAN antibody
      • ACC alpha antibody
      • ACC-alpha antibody
      • ACC1 antibody
      • ACCA antibody
      • Acetyl Coenzyme A antibody
      • Biotin carboxylase antibody
      see all

    画像

    • Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
      Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

      Acetyl Coenzyme A carboxylase alpha was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell), whole cell lysate 10 µg with ab269272 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab269272 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: 293T whole cell lysate 10 µg.

      Lane 2: ab269272 IP in 293T whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269272 in 293T whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 8 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

    • Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
      Immunoprecipitation - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

      Acetyl Coenzyme A carboxylase alpha was immunoprecipitated from 0.35 mg C2C12 (mouse myoblasts myoblast), whole cell lysate 10 µg with ab269272 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab269272 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: C2C12 whole cell lysate 10 µg.

      Lane 2: ab269272 IP in C2C12 whole cell lysate 10 µg.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269272 in C2C12 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 8 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

    • Flow Cytometry - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
      Flow Cytometry - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) cells labelling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

    • Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
      Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T cells labelling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplasmic staining in 293T cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 µg/ml dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

    • Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
      Immunocytochemistry/ Immunofluorescence - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 cells labelling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 µg/ml dilution (Green). Confocal image showing cytoplasmic staining in C2C12 cell line. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 µg/ml dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 µg/ml dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

      Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat lung (PMID: 17521700). The section was incubated with ab269272 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

      Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse lung (PMID: 17521700). The section was incubated with ab269272 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

      Immunohistochemical analysis of paraffin-embedded human endometrial carcinoma tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human endometrial carcinoma. The section was incubated with ab269272 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

      Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Acetyl Coenzyme A carboxylase alpha with ab269272 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human breast carcinoma (PMID: 21415164). The section was incubated with ab269272 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab269272).

    • Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)
      Anti-Acetyl Coenzyme A carboxylase alpha antibody [EPR23235-47] - BSA and Azide free (ab272703)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

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