Name: Anti-ACE2 antibody [EPR4436]
Brand: RabMAb
SKU: ab108209
Rating: 5 (7 reviews)

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Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4436] to ACE2
Suitable for: WB, IP, IHC-P
Knockout validated
Reacts with: Human

Carrier Free

製品名

Anti-ACE2 antibody [EPR4436]
ACE2 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR4436] to ACE2
由来種

Rabbit
アプリケーション

適用あり: WB, IP, IHC-Pmore details
適用なし: Flow Cyt or ICC
種交差性

交差種: Human
非交差種: Mouse
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: Human testis, kidney and lung lysates; Human fetal kidney lysates; Calu-3, HepG2 and Caco-2 cell lysates. IHC-P: Human kidney and testis tissue. IP: Human testis tissue lysate.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR4436
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-ACE2 antibody [EPR4436] - BSA and Azide free (ab247610)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human ACE2 knockout Hep G2 cell line (ab273733)
KO cell lysates
- Human ACE2 knockout Hep G2 cell lysate (ab275495)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab108209の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB	(3)	1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
IP		1/10 - 1/100.
IHC-P	(1)	1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98°C, below boiling, and then let cool for 10-20 minutes. The use of an HRP/AP polymerized secondary antibody is recommended.

特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 92 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98°C, below boiling, and then let cool for 10-20 minutes. The use of an HRP/AP polymerized secondary antibody is recommended.

追加情報

Is unsuitable for Flow Cyt or ICC.

機能

Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses.
組織特異性

Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells. Expressed in lung alveolar epithelial cells, enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system.
配列類似性

Belongs to the peptidase M2 family.
翻訳後修飾

N-glycosylation on Asn-90 may limit SARS infectivity.
細胞内局在

Secreted and Cell membrane.
Target information above from: UniProt accession Q9BYF1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 59272 Human
- Omim: 300335 Human
- SwissProt: Q9BYF1 Human
- Unigene: 178098 Human
別名
- ACE 2 antibody
- ACE related carboxypeptidase antibody
- ACE-related carboxypeptidase antibody
- ACE2 antibody
- ACE2_HUMAN antibody
- ACEH antibody
- Angiotensin converting enzyme 2 antibody
- Angiotensin converting enzyme homolog antibody
- Angiotensin converting enzyme like protein antibody
- Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 antibody
- Angiotensin I converting enzyme 2 antibody
- Angiotensin-converting enzyme homolog antibody
- DKFZP434A014 antibody
- EC 3.4.17 antibody
- metalloprotease MPROT 15 antibody
- Metalloprotease MPROT15 antibody
- OTTHUMP00000022963 antibody
- Processed angiotensin-converting enzyme 2 antibody
see all

Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)

All lanes : Anti-ACE2 antibody [EPR4436] (ab108209) at 1/1000 dilution

Lane 1 : Wild-type HepG2 cell lysate
Lane 2 : ACE2 knockout HepG2 cell lysate
Lane 3 : Calu-3 cell lysate
Lane 4 : A549 cell lysate

Lysates/proteins at 30 µg per lane.

Performed under reducing conditions.

Predicted band size: 92 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab108209 observed at 130 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab108209 was shown to react with ACE2 in wild-type HepG2 cells in western blot with loss of signal observed in ACE2 knockout cell line ab273733 (knockout cell lysate ab275495). Wild-type and ACE2 knockout HepG2 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab108209 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4436] (ab108209)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling ACE2 with ab108209 at 1/100. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)

All lanes : Anti-ACE2 antibody [EPR4436] (ab108209) at 1/1000 dilution

Lane 1 : Wild-type Caco-2 cell lysate
Lane 2 : ACE2 knockout Caco-2 cell lysate
Lane 3 : Calu-3 cell lysate
Lane 4 : A549 cell lysate

Lysates/proteins at 30 µg per lane.

Performed under reducing conditions.

Predicted band size: 92 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?

Lanes 1 - 4: Merged signal (red and green). Green - ab108209 observed at 125 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab108209 was shown to react with ACE2 in Caco-2 wild-type cells in western blot with loss of signal observed in ACE2 knockout cell line ab273731 (knockout cell lysate ab275516). Wild-type and ACE2 knockout Caco-2 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab108209 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)

All lanes : Anti-ACE2 antibody [EPR4436] (ab108209) at 1/1000 dilution

Lane 1 : Human testis cell lysate
Lane 2 : Human kidney cell lysate
Lane 3 : Human lung cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Caco-2 cell lysate
Lane 6 : A549 cell lysate (negative control)

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 92 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?

Lanes 1 - 6: Merged signal (red and green). Green - ab108209 observed at 120 kDa. Red - loading control, Mouse anti-Actin observed at 42kDa.

ab108209 was shown to react with ACE2 in western blot. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab108209 and Mouse anti-Actin overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

Absence of ACE2 expression in A549 cells aligns with previously reported mRNA and protein data (PMID 16282461; fig.2b and 2c).
Western blot - Anti-ACE2 antibody [EPR4436] (ab108209)

All lanes : Anti-ACE2 antibody [EPR4436] (ab108209)

Lane 1 : Human fetal kidney lysate
Lane 2 : Human testis lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 92 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?
Immunoprecipitation - Anti-ACE2 antibody [EPR4436] (ab108209)

ab108209 Immunoprecipitating ACE2 in human testis tissue lysate. 0.35 mg of tissue lysate was incubated with 2 μg primary antibody (1/30). For western blotting a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1000) was used to confirm successful immunoprecipitation.

Exposure time: 1 second.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.

All lanes : Anti-ACE2 antibody [EPR4436] (ab108209) at 1/500 dilution

Lane 1 : Human testis tissue lysate at 10 µg
Lane 2 : ab108209 + Human testis tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab108209 in Human testis tissue lysate

Observed band size: 110 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACE2 antibody [EPR4436] (ab108209)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling ACE2 with ab108209 at 1/100. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Anti-ACE2 antibody [EPR4436] (ab108209)

Western blot protocols
Immunoprecipitation protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

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Datasheet download

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ab108209 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108209 は 9 報の論文で使用されています。

Zhang Q et al. Angiotensin-converting Enzyme 2-containing Small Extracellular Vesicles and Exomeres Bind the Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein. Gastroenterology 160:958-961.e3 (2021). PubMed: 33022277
Huang KY et al. Humanized COVID-19 decoy antibody effectively blocks viral entry and prevents SARS-CoV-2 infection. EMBO Mol Med 13:e12828 (2021). PubMed: 33159417
Winstone H et al. The Polybasic Cleavage Site in SARS-CoV-2 Spike Modulates Viral Sensitivity to Type I Interferon and IFITM2. J Virol 95:N/A (2021). PubMed: 33563656
Fu Y & Xiong S Tagged extracellular vesicles with the RBD of the viral spike protein for delivery of antiviral agents against SARS-COV-2 infection. J Control Release 335:584-595 (2021). PubMed: 34089793
Rawle DJ et al. ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactions. PLoS Pathog 17:e1009723 (2021). PubMed: 34214142
Mao Y et al. The Adrenal Cortex, an Underestimated Site of SARS-CoV-2 Infection. Front Endocrinol (Lausanne) 11:593179 (2020). PubMed: 33488517
Liang Y et al. Role of neutrophil chemoattractant CXCL5 in SARS-CoV-2 infection-induced lung inflammatory innate immune response in an in vivo hACE2 transfection mouse model. Zool Res 41:621-631 (2020). PubMed: 33045777
Sun SH et al. A Mouse Model of SARS-CoV-2 Infection and Pathogenesis. Cell Host Microbe 28:124-133.e4 (2020). PubMed: 32485164
Pellegrini L et al. SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids. Cell Stem Cell 27:951-961.e5 (2020). PubMed: 33113348

レビューと Q&A

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レビューを送る質問を送る

1-7 of 7 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Mice hepatocytes)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

Mice hepatocytes

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Formaldehyde

DR. Armen Petrosyan

Verified customer

投稿 Aug 18 2020

Application

Western blot

Sample

Mouse Cell lysate - whole cell (Mice hepatocytes)

Gel Running Conditions

Reduced Denaturing (8% SDS-PAGE)

Loading amount

40 µg

Specification

Mice hepatocytes

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

投稿 Jul 29 2020

Application

Western blot

Sample

Rat Cell lysate - whole cell (Rat hepatocytes)

Gel Running Conditions

Reduced Denaturing (8% SDS-PAGE)

Loading amount

30 µg

Specification

Rat hepatocytes

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

投稿 Jul 27 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Human Alveolar Epithelial Cells)

Permeabilization

Yes - 0.2% Triton X-100 in PBS

Specification

Human Alveolar Epithelial Cells

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Fixative

Formaldehyde

DR. Armen Petrosyan

Verified customer

投稿 Jul 02 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Kidney)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH6 NaCitrate

Permeabilization

Specification

Kidney

Blocking step

H2O2 as blocking agent for 7 minute(s) · Concentration: 3% · Temperature: 24°C

Fixative

Formaldehyde

In Hwa Um

Verified customer

投稿 Jun 26 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Specification

HeLa

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

投稿 May 21 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (HeLa)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

HeLa

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C

DR. Jerome Cattin

Verified customer

投稿 May 21 2020

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Key features and details

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製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

KO cell lines

KO cell lysates

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

配列類似性

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (9)

レビューと Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-ACE2 antibody [EPR4436]

Western blot abreview for Anti-ACE2 antibody [EPR4436]

Western blot abreview for Anti-ACE2 antibody [EPR4436]

Immunocytochemistry/ Immunofluorescence abreview for Anti-ACE2 antibody [EPR4436]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ACE2 antibody [EPR4436]

Immunocytochemistry/ Immunofluorescence abreview for Anti-ACE2 antibody [EPR4436]

Western blot abreview for Anti-ACE2 antibody [EPR4436]