Anti-ACADM/MCAD 抗体 [EPR3707] - BSA and Azide free (ab247603)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3707] to ACADM/MCAD - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ACADM/MCAD antibody [EPR3707] - BSA and Azide free
ACADM/MCAD 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3707] to ACADM/MCAD - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details
適用なし: Flow Cyt -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab247603 is the carrier-free version of ab108192.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3707 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab247603の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For antigen retrieval, heat up to 98 degree C, below boiling, and then let cool for 10-20 minutes.
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WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa. |
ターゲット情報
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機能
This enzyme is specific for acyl chain lengths of 4 to 16. -
パスウェイ
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
関連疾患
Defects in ACADM are the cause of acyl-CoA dehydrogenase medium-chain deficiency (ACADMD) [MIM:201450]. It is an autosomal recessive disease which causes fasting hypoglycemia, hepatic dysfunction, and encephalopathy, often resulting in death in infancy. -
配列類似性
Belongs to the acyl-CoA dehydrogenase family. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 34 Human
- Entrez Gene: 11364 Mouse
- Entrez Gene: 24158 Rat
- Omim: 607008 Human
- SwissProt: P11310 Human
- SwissProt: P45952 Mouse
- SwissProt: P08503 Rat
- Unigene: 445040 Human
see all -
別名
- ACAD 1 antibody
- ACAD1 antibody
- Acadm antibody
see all
画像
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All lanes : Anti-ACADM/MCAD antibody [EPR3707] (ab108192) at 1/1000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 47 kDaThis data was developed using ab108192, the same antibody clone in a different buffer formulation.
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This data was developed using ab108192, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of ACADM/MCAD in paraffin embedded Human kidney tissue, using ab108192 at a 1/250 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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This data was developed using ab108192, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of ACADM/MCAD in paraffin embedded Human liver tissue, using ab108192 at a 1/250 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab247603 は論文での使用が確認できていません。