Key features and details
- Purity: > 85% HPLC
- Suitable for: MS, Functional Studies
製品名Ac-TVASSSTA Octapeptide peptide
精製度> 85 % HPLC.
Our Abpromise guarantee covers the use of ab95132 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 2.38% HEPES, 0.58% Sodium chloride
再構成Hydrate in 0.5ml of H2O.
関連性Ac-TVASSSTA is an Octapeptide mimic of the N-terminal residues of the reactive center loop of PAI-1 (cf. P14-P7 residues). The Octapeptide inactivates PAI-1 by substituting for strand 4 in beta-sheet A in a manner that competes with formation of the latent species. Insertion of the peptide into beta-sheet A effectively forms a stable complex that converts PAI-1 into a substrate for tissue-type plasminogen activator (tPA) and other target proteinases. The resulting species of PAI-1 is thermodynamically stable and is useful for investigating the role of reactive center loop insertion.
Mass spectrometry analysis of ab95132
HPLC analysis of ab95132
10% SDS-PAGE Reduced
Lane 1: PAI-1 (5 ug) Frozen Lane 2: PAI-1 (5 ug) Incubated
Lane 3: PAI-1 (5 ug) + ab95132 (3 ug) Incubated Lane 4: PAI-1 (5 ug) Frozen + tPA (0.07 ug)
Lane 5: PAI-1 (5 ug) Incubated + tPA (0.07 ug) Lane 6: PAI-1 (5 ug) + ab95132 (3 ug) Incubated + tPA (0.07 ug)
Lane 7: Molecular weight markers
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.