Anti-ABL1 (phospho Y412) 抗体 (ab4717)

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Western blot - Anti-ABL1 (phospho Y412) antibody (ab4717)

    Key features and details

    • Rabbit polyclonal to ABL1 (phospho Y412)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-ABL1 (phospho Y412) antibody
      ABL1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to ABL1 (phospho Y412)

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse

    • 免疫原

      Synthetic peptide corresponding to Human ABL1 (phospho Y412).

    • ポジティブ･コントロール

      • Fibroblasts transfected with oncogenic ∆SH3-Abl.

    • 特記事項

      c-Abl is a 140-150 kDa non-receptor protein tyrosine kinase whose precise functions are not known, but roles for Abl in growth factor and integrin signaling, cell cycle regulation, cytoskeletal reorganization, neurogenesis, and responses to DNA damage and oxidative stress have been suggested. c-Abl kinase activity is increased in vivo by diverse physiological stimuli including ionizing radiation, entry into S phase, integrin activation, and platelet-derived growth factor (PDGF) stimulation. c-Abl contains various protein binding domains that appear to enable it to regulate the functions of many proteins by forming complexes, most notably three isoforms of the oncogenic protein Bcr/Abl. c-Abl becomes fully activated by sequential phosphorylation of tyrosines 412 and 245.

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    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      pH: 7.30
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 0.1% BSA

      PBS is without Mg2+ and Ca2+ and BSA is IgG and protease free.
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively pre-adsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated c-Abl. The final product is generated by affinity chromatography using a c-Abl derived peptide that is phosphorylated at tyrosine 245.

    • 一次抗体 備考

      c-Abl is a 140-150 kDa non-receptor protein tyrosine kinase whose precise functions are not known, but roles for Abl in growth factor and integrin signaling, cell cycle regulation, cytoskeletal reorganization, neurogenesis, and responses to DNA damage and oxidative stress have been suggested. c-Abl kinase activity is increased in vivo by diverse physiological stimuli including ionizing radiation, entry into S phase, integrin activation, and platelet-derived growth factor (PDGF) stimulation. c-Abl contains various protein binding domains that appear to enable it to regulate the functions of many proteins by forming complexes, most notably three isoforms of the oncogenic protein Bcr/Abl. c-Abl becomes fully activated by sequential phosphorylation of tyrosines 412 and 245.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab4717の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 140 kDa.
    特記事項
    WB
    Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 140 kDa.

    ターゲット情報

    • 機能

      Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.

    • 組織特異性

      Widely expressed.

    • 関連疾患

      Note=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).

    • 配列類似性

      Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.

    • 翻訳後修飾

      Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
      Isoform IB is myristoylated on Gly-2.

    • 細胞内局在

      Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Abelson murine leukemia viral oncogene homolog 1 antibody
        • Abelson tyrosine protein kinase 1 antibody
        • Abl 1 antibody
        • ABL antibody
        • ABL proto oncogene 1 non receptor tyrosine kinase antibody
        • ABL1 antibody
        • ABL1_HUMAN antibody
        • bcr/abl antibody
        • bcr/c abl oncogene protein antibody
        • c ABL antibody
        • c abl oncogene 1 non receptor tyrosine kinase antibody
        • c abl oncogene 1 receptor tyrosine kinase antibody
        • c ABL1 antibody
        • JTK7 antibody
        • p150 antibody
        • Proto oncogene tyrosine protein kinase ABL1 antibody
        • Proto-oncogene c-Abl antibody
        • Tyrosine-protein kinase ABL1 antibody
        • v abl Abelson murine leukemia viral oncogene homolog 1 antibody
        • v abl antibody
        see all

      画像

      • Western blot - Anti-ABL1 (phospho Y412) antibody (ab4717)
        Western blot - Anti-ABL1 (phospho Y412) antibody (ab4717)

        Peptide Competition:
        Fibroblasts transfected with oncogenic ∆SH3-Abl were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL phospho c-Abl (Tyr 412) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to phospho c-Abl (Tyr 412) blocks the antibody signal, thereby demonstrating the specificity of the antibody.

        Peptide Competition: Fibroblasts transfected with oncogenic ?SH3-Abl were resolved by SDS-PAGE on a 10%

      参考文献 (8)

      ab4717 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab4717 は 8 報の論文で使用されています。

      • Zhou HJ  et al. Caveolae-mediated Tie2 signaling contributes to CCM pathogenesis in a brain endothelial cell-specific Pdcd10-deficient mouse model. Nat Commun 12:504 (2021). PubMed: 33495460
      • Pandolfi L  et al. Loading Imatinib inside targeted nanoparticles to prevent Bronchiolitis Obliterans Syndrome. Sci Rep 10:20726 (2020). PubMed: 33244143
      • McDaniel NK  et al. AXL Mediates Cetuximab and Radiation Resistance Through Tyrosine 821 and the c-ABL Kinase Pathway in Head and Neck Cancer. Clin Cancer Res 26:4349-4359 (2020). PubMed: 32439698
      • Codullo V  et al. Imatinib-loaded gold nanoparticles inhibit proliferation of fibroblasts and macrophages from systemic sclerosis patients and ameliorate experimental bleomycin-induced lung fibrosis. J Control Release 310:198-208 (2019). PubMed: 31430501
      • Latella L  et al. DNA damage signaling mediates the functional antagonism between replicative senescence and terminal muscle differentiation. Genes Dev 31:648-659 (2017). PubMed: 28446595
      • Kim SY  et al. Rescue of platinum-damaged oocytes from programmed cell death through inactivation of the p53 family signaling network. Cell Death Differ 20:987-97 (2013). PubMed: 23598363
      • Furlan A  et al. Abl interconnects oncogenic Met and p53 core pathways in cancer cells. Cell Death Differ 18:1608-16 (2011). PubMed: 21455220
      • Stevens TL  et al. Using bcr-abl to examine mechanisms by which abl kinase regulates morphogenesis in Drosophila. Mol Biol Cell 19:378-93 (2008). WB . PubMed: 17959833

      レビューと Q&A

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      Question

      Customer would like to know the immunogen sequence.

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      Answer

      Thank you for your enquiry. The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Abl 1b that contains tyrosine 412. The immunogen sequence is approximately 10 amino acid residues. The immunogen would have the phosphorylation site in the middle of the sequence with around 5 aa on one side of the site, and 5 aa on the other side of the site. The exact sequence is considered proprietary. If you have any more questions, please contact us again.

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