リコンビナントRabMAb

Anti-ABL1 抗体 [EPR23406-32] - BSA and Azide free (ab272701)

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Flow Cytometry - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (ab272701)
  • Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (ab272701)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23406-32] to ABL1 - BSA and Azide free
  • Suitable for: IP, Flow Cyt, WB
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free
    ABL1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23406-32] to ABL1 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: K-562, MCF7 and Daudi whole cell lysate. Flow Cyt: K-562 cells.

  • 特記事項

    ab272701 is the carrier-free version of ab254341. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272701 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
アプリケーション Abreviews 特記事項
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
特記事項
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
  • 追加情報
    Is unsuitable for ICC/IF or IHC-P.

    • ターゲット情報

    • 機能

      Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks.

    • 組織特異性

      Widely expressed.

    • 関連疾患

      Note=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL).

    • 配列類似性

      Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.

    • 翻訳後修飾

      Phosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
      Isoform IB is myristoylated on Gly-2.

    • 細胞内局在

      Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Abelson murine leukemia viral oncogene homolog 1 antibody
        • Abelson tyrosine protein kinase 1 antibody
        • Abl 1 antibody
        • ABL antibody
        • ABL proto oncogene 1 non receptor tyrosine kinase antibody
        • ABL1 antibody
        • ABL1_HUMAN antibody
        • bcr/abl antibody
        • bcr/c abl oncogene protein antibody
        • c ABL antibody
        • c abl oncogene 1 non receptor tyrosine kinase antibody
        • c abl oncogene 1 receptor tyrosine kinase antibody
        • c ABL1 antibody
        • JTK7 antibody
        • p150 antibody
        • Proto oncogene tyrosine protein kinase ABL1 antibody
        • Proto-oncogene c-Abl antibody
        • Tyrosine-protein kinase ABL1 antibody
        • v abl Abelson murine leukemia viral oncogene homolog 1 antibody
        • v abl antibody
        see all

      画像

      • Flow Cytometry - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (ab272701)
        Flow Cytometry - Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (ab272701)

        Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized K-562 (Human chronic myelogenous leukemia lymphoblast) cells labelling ABL1 with ab254341 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254341).

      • Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (ab272701)
        Anti-ABL1 antibody [EPR23406-32] - BSA and Azide free (ab272701)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab272701 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab272701 は論文での使用が確認できていません。

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