Rabbit polyclonal to ABI3BP
Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to a region within N terminal amino acids 143-192 (LINPHHDWTL PSHCPNDRFY TIRYREKDKE KKWIFQICPA TETIVENLKP) of Human ABI3BP (NP_056244)
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Predicted molecular weight: 119 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
Expressed in brain, heart, lung, liver, pancreas kidney and placenta.
Contains 2 fibronectin type-III domains.
Information by UniProt
- ABI gene family member 3 (NESH) binding protein antibody
- ABI gene family member 3 binding protein antibody
- ABI gene family member 3-binding protein antibody
Western blot - Anti-ABI3BP antibody (ab82802)
Anti-ABI3BP antibody (ab82802) at 1 µg/ml + HeLa cell lysate at 10 µgSecondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilutionPredicted band size:
119 kDaObserved band size:
145 kDa (why is the actual band size different from the predicted?
)Additional bands at:
20 kDa, 35 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 6-18%
has not yet been referenced specifically in any publications.
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