Anti-Abi-1 (phospho Y435) 抗体 [EP3954] (ab109395)
![Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] (ab109395)](https://www.abcam.co.jp/ps/products/109/ab109395/Images/SSH3BP1-Primary-antibodies-ab109395-2.jpg "Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] (ab109395)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP3954] to Abi-1 (phospho Y435)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Abi-1 (phospho Y435) antibody [EP3954]
Abi-1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP3954] to Abi-1 (phospho Y435) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Jurkat cell lysate treated with pervanadate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP3954 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109395の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 55 kDa.
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| 特記事項 |
|---|
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WB
1/10000 - 1/50000. Predicted molecular weight: 55 kDa. |
追加情報
Is unsuitable for IHC-P.
ターゲット情報
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機能
May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. -
組織特異性
Widely expressed, with highest expression in brain. -
関連疾患
Note=A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with MLL. ABI1 isoform 2 was found to be present in acute leukemia MLL-ABI1 fusion transcript. -
配列類似性
Belongs to the ABI family.
Contains 1 SH3 domain.
Contains 1 t-SNARE coiled-coil homology domain. -
ドメイン
The t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A. -
翻訳後修飾
In vitro substrate for v-Abl (By similarity). Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl. -
細胞内局在
Cytoplasm. Nucleus. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes. - Information by UniProt
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参照データベース
- Entrez Gene: 10006 Human
- Omim: 603050 Human
- SwissProt: Q8IZP0 Human
- Unigene: 508148 Human
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別名
- Abelson interactor 1 antibody
- Abi-1 antibody
- Abi1 antibody
see all
画像
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Western blot - Anti-Abi-1 (phospho Y435) antibody [EP3954] (ab109395)All lanes : Anti-Abi-1 (phospho Y435) antibody [EP3954] (ab109395) at 1/10000 dilution
Lane 1 : Jurkat cell lysate, untreated
Lane 2 : Jurkat cell lysate, treated with pervanadate
Lysates/proteins at 10 µg per lane.
Predicted band size: 55 kDa -
![Anti-Abi-1 (phospho Y435) antibody [EP3954] (ab109395)](https://www.abcam.co.jp/ps/products/109/ab109395/Images/ab109395-2-benefits-of-recombinant-antibodies.png)
Anti-Abi-1 (phospho Y435) antibody [EP3954] (ab109395)
データシートおよび資料
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Datasheet download
参考文献 (0)
ab109395 は論文での使用が確認できていません。
