Anti-ABCG8 抗体 (ab223056)
Key features and details
- Rabbit polyclonal to ABCG8
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-ABCG8 antibody
ABCG8 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ABCG8 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment corresponding to Human ABCG8 aa 176-413.
Database link: Q9H221 -
ポジティブ・コントロール
- WB: Mouse heart, lung, kidney and stomach lysates. IHC-P: Human lung tissue. ICC/IF: MCF7 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purity >95% -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab223056の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa).
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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特記事項 |
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WB
1/2000 - 1/10000. Detects a band of approximately 76 kDa (predicted molecular weight: 76 kDa). |
IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
ターゲット情報
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機能
Transporter that appears to play an indispensable role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. -
組織特異性
Strongly expressed in the liver, lower levels in the small intestine and colon. Detectable in a wide variety of human tissues. -
関連疾患
Genetic variations in ABCG8 can be associated with susceptibility to gallbladder disease type 4 (GBD4) [MIM:611465]. With an overall prevalence of 10-20%, gallstone disease (cholelithiasis) represents one of the most frequent and economically relevant health problems of industrialized countries.
Defects in ABCG8 are a cause of sitosterolemia (STSL) [MIM:210250]; also known as phytosterolemia or shellfish sterolemia. It is a rare autosomal recessive disorder characterized by increased intestinal absorption of all sterols including cholesterol, plant and shellfish sterols, and decreased biliary excretion of dietary sterols into bile. Sitosterolemia patients have hypercholesterolemia, very high levels of plant sterols in the plasma, and frequently develop tendon and tuberous xanthomas, accelerated atherosclerosis and premature coronary artery disease. -
配列類似性
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 64241 Human
- Entrez Gene: 67470 Mouse
- Omim: 605460 Human
- SwissProt: Q9H221 Human
- SwissProt: Q9DBM0 Mouse
- Unigene: 413931 Human
- Unigene: 26581 Mouse
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別名
- ABCG8 antibody
- ABCG8_HUMAN antibody
- ATP binding cassette sub family G (WHITE) member 8 (sterolin 2) antibody
see all
画像
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All lanes : Anti-ABCG8 antibody (ab223056) at 1/2000 dilution
Lane 1 : Mouse heart lysate
Lane 2 : Mouse lung lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse stomach lysate
Secondary
All lanes : Goat Anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 76 kDa
Observed band size: 76 kDa -
Paraffin-embedded human lung tissue stained for ABCG8 using ab223056 at 1/100 dilution in immunohistochemical analysis.
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MCF7 (human breast adenocarcinoma cell line) cells stained for ABCG8 (green) using ab223056 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG(H+L).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab223056 は 2 報の論文で使用されています。
- Zhang Z et al. HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARa axis. Mol Ther Nucleic Acids 24:711-727 (2021). PubMed: 33996254
- Huang X et al. [Lipid-lowering effect of propolis in mice with Triton-WR1339-induced hyperlipidemia and its mechanism for regulating lipid metabolism]. Nan Fang Yi Ke Da Xue Xue Bao 38:1020-1024 (2018). PubMed: 30187881