リコンビナントRabMAb

Anti-ABCB4 抗体 [EPR23697-35] (ab272457)

Submit a review Submit a question
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB4 antibody [EPR23697-35] (ab272457)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB4 antibody [EPR23697-35] (ab272457)
  • Anti-ABCB4 antibody [EPR23697-35] (ab272457)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23697-35] to ABCB4
  • Suitable for: IHC-P
  • Reacts with: Human

製品の概要

  • 製品名

    Anti-ABCB4 antibody [EPR23697-35]
    ABCB4 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23697-35] to ABCB4

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: Human liver, Human skeletal muscle tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Mouse
Human
ICC
Mouse
Human
IHC-P
Human
IP
Mouse
Human
WB
Rat
Human
アプリケーション Abreviews 特記事項
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
特記事項
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF,IP or WB.

    • ターゲット情報

    • 機能

      Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human MDR3 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.

    • 関連疾患

      Defects in ABCB4 are the cause of progressive familial intrahepatic cholestasis type 3 (PFIC3) [MIM:602347]. PFIC3 is an autosomal recessive liver disorder presenting with early onset cholestasis that progresses to cirrhosis and liver failure before adulthood. It is characterized by elevated serum gamma-glutamyltransferase levels.
      Defects in ABCB4 are a cause of intrahepatic cholestasis of pregnancy (ICP) [MIM:147480]; also known as obstetric cholestasis. ICP is a multifactorial liver disorder of pregnancy. It presents during the second or, more commonly, the third trimestre of pregnancy with intense pruritus which becomes more severe with advancing gestation and cholestasis. Cholestasis results from abnormal biliary transport from the liver into the small intestine. ICP causes fetal distress, spontaneous premature delivery and intrauterine death. ICP patients have spontaneous and progressive disappearance of cholestasis after delivery.
      Defects in ABCB4 are a cause of gallbladder disease type 1 (GBD1) [MIM:600803]. It is one of the major digestive diseases. Gallstones composed of cholesterol (cholelithiasis) are the common manifestations in western countries. Most people with gallstones, however, remain asymptomatic through their lifetimes.

    • 配列類似性

      Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily.
      Contains 2 ABC transmembrane type-1 domains.
      Contains 2 ABC transporter domains.

    • 細胞内局在

      Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • ABC 21 antibody
        • ABC B4 antibody
        • ABC21 antibody
        • ABCB 4 antibody
        • Abcb4 antibody
        • ABCB4 protein antibody
        • ATP binding cassette sub family B MDR/TAP member 4 antibody
        • ATP binding cassette sub family B member 4 antibody
        • ATP-binding cassette sub-family B member 4 antibody
        • GBD1 antibody
        • ICP3 antibody
        • MDR 3 antibody
        • MDR2 antibody
        • MDR2/3 antibody
        • MDR3 antibody
        • MDR3 P glycoprotein antibody
        • MDR3 P gp antibody
        • MDR3_HUMAN antibody
        • Multidrug resistance protein 3 antibody
        • Multiple drug resistance 3 antibody
        • P glycoprotein 3 antibody
        • P-glycoprotein 3 antibody
        • PFIC 3 antibody
        • PFIC3 antibody
        • PGY 3 antibody
        • PGY3 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB4 antibody [EPR23697-35] (ab272457)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB4 antibody [EPR23697-35] (ab272457)

        Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ABCB4 with ab272457 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining in human skeletal muscle (PMID: 7734012).The section was incubated with ab272457 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB4 antibody [EPR23697-35] (ab272457)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCB4 antibody [EPR23697-35] (ab272457)

        Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ABCB4 with ab272457 at 1/4000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in bile canaliculi of human liver (PMID:24594635). The section was incubated with ab272457 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

      • Anti-ABCB4 antibody [EPR23697-35] (ab272457)
        Anti-ABCB4 antibody [EPR23697-35] (ab272457)

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab272457 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab272457 は論文での使用が確認できていません。

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      There are currently no Customer reviews or Questions for ab272457.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com