Application
Western blot
Sample
Pig Tissue lysate - whole (small intestine)
Loading amount
30 µg
Specification
small intestine
Gel Running Conditions
Reduced Denaturing (7)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse HRP conjugated secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 254 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Dongil Kim
Verified customer
投稿 Apr 23 2013