Name: Anti-ABCA1 antibody [AB.H10]
SKU: ab18180
Rating: 4 (17 reviews)

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Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (ab18180)

Key features and details

Mouse monoclonal [AB.H10] to ABCA1
Suitable for: IHC-P, Flow Cyt, WB
Knockout validated
Reacts with: Mouse, Human
Isotype: IgG1

製品名

Anti-ABCA1 antibody [AB.H10]
ABCA1 一次抗体製品一覧
製品の詳細

Mouse monoclonal [AB.H10] to ABCA1
由来種

Mouse
アプリケーション

適用あり: IHC-P, Flow Cyt, WBmore details
種交差性

交差種: Mouse, Human
免疫原

Recombinant fragment corresponding to Human ABCA1 aa 1800-2260.
Database link: O95477
ポジティブ･コントロール
- testis, liver, and brain tissue (negative control: muscle tissue)
特記事項

This product was changed from ascites to tissue culture supernatant on 5^th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファー

pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: PBS
Concentration information loading...
精製度

Protein A purified
特記事項（精製）

Protein A purified from TCS
ポリ/モノ

モノクローナル
クローン名

AB.H10
アイソタイプ

IgG1
研究分野
- Neuroscience
- Processes

Compatible Secondaries
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
- Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
Recombinant Protein
- Recombinant Human ABCA1 protein (ab125995)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab18180の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
IHC-P		1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt	(3)	Use 1-2µg for 10⁶ cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB	(12)	1/200 - 1/500. Predicted molecular weight: 254 kDa. Abcam recommends using BSA as the blocking agent and 80-100 ug of lysate.

特記事項
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use 1-2µg for 10⁶ cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/200 - 1/500. Predicted molecular weight: 254 kDa. Abcam recommends using BSA as the blocking agent and 80-100 ug of lysate.

機能

cAMP-dependent and sulfonylurea-sensitive anion transporter. Key gatekeeper influencing intracellular cholesterol transport.
組織特異性

Widely expressed, but most abundant in macrophages.
関連疾患

Defects in ABCA1 are a cause of high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by absence of high density lipoprotein (HDL) cholesterol from plasma, accumulation of cholesteryl esters, premature coronary artery disease (CAD), hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness.
Defects in ABCA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). HDLD2 is inherited as autosomal dominant trait. It is characterized by moderately low HDL cholesterol, predilection toward premature coronary artery disease (CAD) and a reduction in cellular cholesterol efflux.
配列類似性

Belongs to the ABC transporter superfamily. ABCA family.
Contains 2 ABC transporter domains.
ドメイン

Multifunctional polypeptide with two homologous halves, each containing an hydrophobic membrane-anchoring domain and an ATP binding cassette (ABC) domain.
翻訳後修飾

Phosphorylation on Ser-2054 regulates phospholipid efflux.
Palmitoylation by DHHC8 is essential for membrane localization.
細胞内局在

Membrane.
Target information above from: UniProt accession O95477 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 19 Human
- Entrez Gene: 11303 Mouse
- Omim: 600046 Human
- SwissProt: O95477 Human
- SwissProt: P41233 Mouse
- Unigene: 429294 Human
- Unigene: 277376 Mouse
別名
- ABC 1 antibody
- ABC Transporter 1 antibody
- ABC-1 antibody
- ABC1 antibody
- ABCA 1 antibody
- ABCA1 antibody
- ABCA1_HUMAN antibody
- ATP binding Cassette 1 antibody
- ATP binding cassette sub family A ABC1 member 1 antibody
- ATP binding cassette sub family A member 1 antibody
- ATP binding Cassette Transporter 1 antibody
- ATP-binding cassette 1 antibody
- ATP-binding cassette sub-family A member 1 antibody
- ATP-binding cassette transporter 1 antibody
- CERP antibody
- Cholesterol efflux regulatory protein antibody
- FLJ14958 antibody
- HDLDT1 antibody
- Membrane bound antibody
- MGC164864 antibody
- MGC165011 antibody
- TD antibody
- TGD antibody
see all

Flow Cytometry - Anti-ABCA1 antibody [AB.H10] (ab18180)

Overlay histogram showing HepG2 cells stained with ab18180 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab18180, 2µg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)

Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous bloting with anti-actin antibody was used for protein loading control. Western blot analysis of ABCA1 induction by cholesterol (+) in human fibroblast cells from different patients (1,2,3,4). Simultaneous blotting with anti-actin antibody was used for protein loading control.
Western blot - Anti-ABCA1 antibody [AB.H10] (ab18180)

All lanes : Anti-ABCA1 antibody [AB.H10] (ab18180) at 1/1000 dilution

Lanes 1 & 3 & 5 : Wild type mouse liver tissue lysate
Lanes 2 & 4 & 6 : Knockout mouse liver tissue lysate

Lysates/proteins at 100 µg per lane.

Predicted band size: 254 kDa
Observed band size: 254 kDa

The lower bands are from the secondary anti-mouse antibody reacting to the endogenous mouse antibodies from the tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [AB.H10] (ab18180)

Immunoreactivity of pancreas tissue for ABCA1 protein. Left panel: strong immunopositivity of exocrine glandular cells of the pancreas, especially in the basal region (arrows). Interstitial microvascular cells are apparently negative. Cells within the islet were weakly positive (data not shown). Right panel: the negative control. Hematoxylin counterstain.

Recommended protocol for IHC-P with ab18180

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab18180 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab18180 は 165 報の論文で使用されています。

Pi S et al. The P2RY12 receptor promotes VSMC-derived foam cell formation by inhibiting autophagy in advanced atherosclerosis. Autophagy 17:980-1000 (2021). PubMed: 32160082
Wanschel ACBA et al. The Presence of Cholesteryl Ester Transfer Protein (CETP) in Endothelial Cells Generates Vascular Oxidative Stress and Endothelial Dysfunction. Biomolecules 11:N/A (2021). PubMed: 33430172
Kim JS et al. Melatonin activates ABCA1 via the BiP/NRF1 pathway to suppress high-cholesterol-induced apoptosis of mesenchymal stem cells. Stem Cell Res Ther 12:114 (2021). PubMed: 33546749
Luo J et al. Ad- and AAV8-mediated ABCA1 gene therapy in a murine model with retinal ischemia/reperfusion injuries. Mol Ther Methods Clin Dev 20:551-558 (2021). PubMed: 33665225
Gnanaguru G et al. Targeting of miR-33 ameliorates phenotypes linked to age-related macular degeneration. Mol Ther 29:2281-2293 (2021). PubMed: 33744470

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-10 of 53 Abreviews or Q&A

Application

Western blot

Sample

Sheep Cell lysate - whole cell (Ovine Thecal Cells)

Gel Running Conditions

Reduced Denaturing (10% PAG)

Loading amount

10 µg

Specification

Ovine Thecal Cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

MISS. Barbara Makela

Verified customer

投稿 Jun 22 2017

Application

Western blot

Sample

Mouse Tissue lysate - whole (liver)

Gel Running Conditions

Reduced Denaturing (7.5)

Loading amount

25 µg

Specification

liver

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Nov 24 2016

Application

Immunocytochemistry/ Immunofluorescence

Sample

Rat Cell (Primary astrocyte culture)

Permeabilization

Yes - 0.1M PBS with 1% Triton X

Specification

Primary astrocyte culture

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C

Fixative

Methanol

DR. Ruma Raha-Chowdhury

Verified customer

投稿 Sep 06 2016

Application

Western blot

Sample

Rabbit Tissue lysate - whole (Synovial membrane)

Gel Running Conditions

Reduced Denaturing (8 %)

Loading amount

35 µg

Specification

Synovial membrane

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Jun 13 2016

Application

Flow Cytometry

Fixation

Formaldehyde

Permeabilization

Yes - BD PERMEABILISATION SOLUTION

Sample

Mouse Cell (liver cells)

Specification

liver cells

Gating Strategy

CD24 POSITIVE

Preparation

Cell harvesting/tissue preparation method: single cell suspension
Sample buffer: 0.1%PBS-BSA

Abcam user community

Verified customer

投稿 Dec 10 2013

Application

Flow Cytometry

Fixation

Formaldehyde

Permeabilization

Yes - BD Permeabilisation Solution

Sample

Rat Cell (Liver cells)

Specification

Liver cells

Gating Strategy

CD24 Positive

Preparation

Cell harvesting/tissue preparation method: Single cell suspension
Sample buffer: 0.1% PBS-BSA

Abcam user community

Verified customer

投稿 Dec 05 2013

Application

Flow Cytometry

Fixation

Formaldehyde

Permeabilization

Yes - BD PERMEABILISATION SOLUTION

Sample

Human Cell (HEP G2)

Specification

HEP G2

Gating Strategy

CD24 POSITIVE

Preparation

Cell harvesting/tissue preparation method: single cell suspension
Sample buffer: 0.1%PBS-BSA

Mr. brahmi manali

Verified customer

投稿 Dec 04 2013

Application

Western blot

Sample

Pig Tissue lysate - whole (small intestine)

Loading amount

30 µg

Specification

small intestine

Gel Running Conditions

Reduced Denaturing (7)

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 20°C

Mr. Dongil Kim

Verified customer

投稿 Apr 23 2013

Question

Thank you for your kind replacement of the antibody! I was able to test it on human prostate cancer cell line PC3 lysed with RIPA buffer (last lane of the samples indicated by arrow) and my other prostate cancer cell lines prepared before. The membrane was first detected with your new antibody and then reprobed with FASN. As you can see from the images, your new antibody can only show a low molecular weight background band. FASN antibody clearly detects the target protein with several non-specific bands. The pictures in two different pages represents different exposure time. Therefore, I am afraid that your new antibody does not work either. If you can provide a positive control, I am willing to repeat experiment again. If your positive control works, it means that your antibodies work. I am happy to keep them and improve my sample preparation.

Abcam community

Verified customer

Asked on Jan 08 2013

Answer

Yes, I can send you as a one time exception a positive control lysate (rat liver (ab29413), https://www.abcam.com/liver-rat-whole-cell-lysate-normal-tissue-ab29413.html). This has been tested with ab66217 and gave strong bands.

Also I would suggest to check the gel after transfer as some of the high MW proteins might have not completely transfered onto the membrane. You can check this by staining the gel with Coomassie Blue. if you see bands in the gel at 200 kDa and higher, you might want to adjust your transfer conditions so that all high MW protein are being transfered.

Also, avoid boiling of your samples before loading them onto the gel. Warming your sample to 50-60 degree Celsius is sufficient for denaturation in loading buffer.

Again, FASN is a cytoplasmic protein and such is much easier to work with than ABCA1 which is very hydrophobic, glycosylated and of high MW.

This article and link might be of help as well: http://www.sciencedirect.com/science/article/pii/S0003269708006829
http://www.scientistsolutions.com/t14685-membrane+protein+western+blot.html

Abcam Scientific Support

Answered on Jan 08 2013

Question

I saw that you changed the pictures from WB of ABAC1 in your datasheet. As you know I didn´t find the protein at , the actual, predicted molecular weigth of 220Kda of the previous 294Kda. My question is, the lower band at +|- 60Kda ( you also found) is correlated with the upper band? The size of the bands are correlated for the same condition?

Abcam community

Verified customer

Asked on Jan 07 2013

Answer

In this image the lower band is actin which has been recognized by and anti-actin antibody. This has been used as a loading control to normalize the levels of protein detected by confirming that protein loading is the same across the gel. The expression levels of the loading control should not vary between the different sample lanes.

Abcam Scientific Support

Answered on Jan 07 2013

1-10 of 53 Abreviews or Q&A

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Key features and details

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

特記事項（精製）

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Compatible Secondaries

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

組織特異性

関連疾患

配列類似性

ドメイン

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (165)

レビューと Q&A

Western blot abreview for Anti-ABCA1 antibody [AB.H10]

Western blot abreview for Anti-ABCA1 antibody [AB.H10]

Immunocytochemistry/ Immunofluorescence abreview for Anti-ABCA1 antibody [AB.H10]

Western blot abreview for Anti-ABCA1 antibody [AB.H10]

Flow Cytometry abreview for Anti-ABCA1 antibody [AB.H10]

Flow Cytometry abreview for Anti-ABCA1 antibody [AB.H10]

Flow Cytometry abreview for Anti-ABCA1 antibody [AB.H10]

Western blot abreview for Anti-ABCA1 antibody [AB.H10]