Anti-S100A9 抗体 [EPR3555] (ab92507)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3555] to S100A9
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-S100A9 antibody [EPR3555]
S100A9 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3555] to S100A9 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details
適用なし: IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Breast cancer, Human tonsil, Human spleen and peripheral blood lymphocytes lysates, Human breast carcinoma tissue and Human spleen tissue.IF: DU145 cell lineFlow Cyt (intra): Jurkat cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3555 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92507の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 13 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. Boil slides seven times for five minutes each in pH 6 citrate buffer.
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ICC/IF |
1/100 - 1/250.
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Flow Cyt (Intra) |
1/200.
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特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 13 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. Boil slides seven times for five minutes each in pH 6 citrate buffer. |
ICC/IF
1/100 - 1/250. |
Flow Cyt (Intra)
1/200. |
ターゲット情報
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機能
Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization when unphosphorylated. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as a pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions. -
組織特異性
Expressed by macrophages in acutely inflammed tissues and in chronic inflammation. Detected in peripheral blood leukocytes, in neutrophils and granulocytes. Detected at sites of vascular inflammation (at protein level). Also expressed in epithelial cells constitutively or induced during dermatoses. -
配列類似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
翻訳後修飾
Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization. -
細胞内局在
Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation. - Information by UniProt
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参照データベース
- Entrez Gene: 6280 Human
- Omim: 123886 Human
- SwissProt: P06702 Human
- Unigene: 112405 Human
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別名
- Leukocyte L1 complex heavy chain antibody
- 60B8AG antibody
- CAGB antibody
see all
画像
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Anti-S100A9 antibody [EPR3555] (ab92507)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling S100A9 with ab92507 at a dilution of 1:500. Heat mediated antigen retrieval was performed using AR9 antigen retrieval solution, and microwave treatment for 15 min at 20% power. Anti-Rabbit/Mouse HRP polymer (PerkinElmer Opal Polymer HRP Ms Plus Rb) was used as secondary antibody. Opal tyramide amplification was performed using Opal 520 fluorophore. Counterstained with DAPI stain. Image scanned with Vectra 3.0 and analyzed via Phenochart software.
This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center. -
ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.
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All lanes : Anti-S100A9 antibody [EPR3555] (ab92507) at 1/1000 dilution
Lane 1 : Breast cancer lysate
Lane 2 : Human tonsil lysate
Lane 3 : Human spleen lysate
Lane 4 : Peripheral blood lymphocytes lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 13 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted? -
Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling S100A9 (red) with purified ab92507 at a 1/200 dilution (10ug/mL). Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
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ab92507, at a 1/250 dilution, staining S100A9 in paraffin embedded Human spleen tissue by Immunohistochemistry.
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ICC/IF image of ab92507 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab92507, 1/200) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgM (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (22)
ab92507 は 22 報の論文で使用されています。
- Zhang H et al. Leaky Gut Driven by Dysbiosis Augments Activation and Accumulation of Liver Macrophages via RIP3 Signaling Pathway in Autoimmune Hepatitis. Front Immunol 12:624360 (2021). PubMed: 33841405
- Chen H et al. NGFR Increases the Chemosensitivity of Colorectal Cancer Cells by Enhancing the Apoptotic and Autophagic Effects of 5-fluorouracil via the Activation of S100A9. Front Oncol 11:652081 (2021). PubMed: 33996571
- Yang Y et al. Proteomic analysis reveals some common proteins in the kidney stone matrix. PeerJ 9:e11872 (2021). PubMed: 34395096
- Yan G et al. Single-cell transcriptomic analysis reveals the critical molecular pattern of UV-induced cutaneous squamous cell carcinoma. Cell Death Dis 13:23 (2021). PubMed: 34934042
- Wang Q et al. Quantitative proteomic analysis of urinary exosomes in kidney stone patients. Transl Androl Urol 9:1572-1584 (2020). PubMed: 32944520