Anti-CaMKII alpha 抗体 [EPR1828] (ab92332)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1828] to CaMKII alpha
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CaMKII alpha antibody [EPR1828]
CaMKII alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR1828] to CaMKII alpha -
由来種
Rabbit -
特異性
The peptide immunogen shares a 100% homology with CaMKII beta, gamma and delta therefore we believe cross-reactivity is highly likely but this has not been experimentally assessed. -
アプリケーション
適用あり: WB, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human CaMKII alpha aa 1-100 (N terminal). The exact sequence is proprietary.
(Peptide available asab175459) -
ポジティブ・コントロール
- Flow Cyt (intra): SH-SY5Y cells WB: rat brain lysate, SH-SY5Y cell lysate, Neuro 2a cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1828 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92332の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/10000 - 1/50000. Predicted molecular weight: 54 kDa.
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Flow Cyt (Intra) |
1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/10000 - 1/50000. Predicted molecular weight: 54 kDa. |
Flow Cyt (Intra)
1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 815 Human
- Entrez Gene: 12322 Mouse
- Entrez Gene: 25400 Rat
- Omim: 114078 Human
- SwissProt: Q9UQM7 Human
- SwissProt: P11798 Mouse
- SwissProt: P11275 Rat
- Unigene: 725992 Human
see all -
別名
- Alpha CaMKII antibody
- Calcium calmodulin dependent protein kinase II antibody
- Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
see all
画像
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Overlay histogram showing SH-SY5Y cells stained with ab92332 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92332, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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All lanes : Anti-CaMKII alpha antibody [EPR1828] (ab92332)
Lane 1 : Rat brain lysate
Lane 2 : SH SY5Y cell lysate
Lane 3 : Neuro 2a cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 54 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (15)
ab92332 は 15 報の論文で使用されています。
- Yeh KC et al. Soybean Meal Extract Preserves Memory Ability by Increasing Presynaptic Function and Modulating Gut Microbiota in Rats. Mol Neurobiol 59:1649-1664 (2022). PubMed: 35001354
- Xu SF et al. Astrocyte-specific loss of lactoferrin influences neuronal structure and function by interfering with cholesterol synthesis. Glia 70:2392-2408 (2022). PubMed: 35946355
- Soleimanpour E et al. Regulation of CREB Phosphorylation in Nucleus Accumbens after Relief Conditioning. Cells 10:N/A (2021). PubMed: 33530478
- Ohnishi T et al. Cooperation of LIM domain-binding 2 (LDB2) with EGR in the pathogenesis of schizophrenia. EMBO Mol Med 13:e12574 (2021). PubMed: 33656268
- Hu X et al. Interplay between Müller cells and microglia aggravates retinal inflammatory response in experimental glaucoma. J Neuroinflammation 18:303 (2021). PubMed: 34952606