Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control (ab91361)
Related conjugates and formulations
製品の概要
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製品名
Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control
Mouse アイソタイプ・コントロール 製品一覧 -
アプリケーション
適用あり: Flow Cytmore details -
免疫原
Synthetic peptide. Hapten from sequence of plant origin.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from supernatant from an in vitro culture. -
ポリ/モノ
モノクローナル -
クローン名
B12/8 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
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別名
- Mouse Isotype Control
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab91361の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use 1µg for 106 cells.
ab91361 is a negative isotype control |
特記事項 |
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Flow Cyt
Use 1µg for 106 cells. ab91361 is a negative isotype control |
画像
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Flow cytometry analysis of normal whole blood sample labeling IgG2a with ab91361 at 1μg.
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Overlay histogram showing A549 cells stained with ab60720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60720, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab91361 は 8 報の論文で使用されています。
- Giadans CG et al. Intrahepatic immune infiltrate in chronic hepatitis B and chronic hepatitis C: Similar but not the same. J Viral Hepat 29:124-134 (2022). PubMed: 34820942
- Benz PM et al. AKAP12 deficiency impairs VEGF-induced endothelial cell migration and sprouting. Acta Physiol (Oxf) 228:e13325 (2020). PubMed: 31162891
- Chen X et al. Oxidative stress-induced IL-15 trans-presentation in keratinocytes contributes to CD8+ T cells activation via JAK-STAT pathway in vitiligo. Free Radic Biol Med 139:80-91 (2019). PubMed: 31078730
- Rios DA et al. Chronic hepatitis C liver microenvironment: role of the Th17/Treg interplay related to fibrogenesis. Sci Rep 7:13283 (2017). PubMed: 29038590
- Khan A et al. Mesenchymal stem cells internalize Mycobacterium tuberculosis through scavenger receptors and restrict bacterial growth through autophagy. Sci Rep 7:15010 (2017). PubMed: 29118429
- Abo Al Hayja M et al. Reduced expression of peroxisome proliferator-activated receptor alpha in BAL and blood T cells of non-löfgren's sarcoidosis patients. J Inflamm (Lond) 12:28 (2015). Flow Cyt ; Human . PubMed: 25969669
- Jun E et al. Design of a multicomponent peptide-woven nanocomplex for delivery of siRNA. PLoS One 10:e0118310 (2015). PubMed: 25705892
- Tashima Y & Stanley P Antibodies that detect O-linked ß-D-N-acetylglucosamine on the extracellular domain of cell surface glycoproteins. J Biol Chem 289:11132-42 (2014). Flow Cyt . PubMed: 24573683