Recombinant human Hsp90 alpha protein (Active) (ab80369)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
製品の詳細
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製品名
Recombinant human Hsp90 alpha protein (Active)
Hsp90 alpha タンパク質・ペプチド 製品一覧 -
生理活性
Kd app= 11.8 nM (lot specific).
Buffer contains 0.1M HEPES, pH 7.4, 0.25M MgCl2, 0.25M KCl, 0.1M Na2MoO4, and 0.05% Triton X. Incubated Hsp90alpha in a series dilution with 5 nM FITC-labeled geldanamycin for 150 minutes at room temperature.
Fluorescence polarization measured at ex 470/em528.
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精製度
>= 90 % SDS-PAGE.
Affinity purified. -
発現系
Escherichia coli -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
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生物種
Human -
配列
MPEETQTQDQ PMEEEEVETF AFQAEIAQLM SLIINTFYSN KEIFLRELIS NSSDALDKIR YESLTDPSKL DSGKELHINL IPNKQDRTLT IVDTGIGMTK ADLINNLGTI AKSGTKAFME ALQAGADISM IGQFGVGFYS AYLVAEKVTV ITKHNDDEQY AWESSAGGSF TVRTDTGEPM GRGTKVILHL KEDQTEYLEE RRIKEIVKKH SQFIGYPITL FVEKERDKEV SDDEAEEKED KEEEKEKEEK ESEDKPEIED VGSDEEEEKK DGDKKKKKKI KEKYIDQEEL NKTKPIWTRN PDDITNEEYG EFYKSLTNDW EDHLAVKHFS VEGQLEFRAL LFVPRRAPFD LFENRKKKNN IKLYVRRVFI MDNCEELIPE YLNFIRGVVD SEDLPLNISR EMLQQSKILK VIRKNLVKKC LELFTELAED KENYKKFYEQ FSKNIKLGIH EDSQNRKKLS ELLRYYTSAS GDEMVSLKDY CTRMKENQKH IYYITGETKD QVANSAFVER LRKHGLEVIY MIEPIDEYCV QQLKEFEGKT LVSVTKEGLE LPEDEEEKKK QEEKKTKFEN LCKIMKDILE KKVEKVVVSN RLVTSPCCIV TSTYGWTANM ERIMKAQALR DNSTMGYMAA KKHLEINPDH SIIETLRQKA EADKNDKSVK DLVILLYETA LLSSGFSLED PQTHANRIYR MIKLGLGIDE DDPTADDTSA AVTEEMPPLE GDDDTSRMEE VDHHHHHH -
予測される分子量
86 kDa including tags -
領域
1 to 732 -
タグ
His tag C-Terminus -
配列の追加情報
(NM_005348)
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関連製品
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Related Products
特性
Our Abpromise guarantee covers the use of ab80369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
SDS-PAGE
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製品の状態
Liquid -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped on Dry Ice. Upon delivery aliquot. Upon reconsitution add a carrier protein (0.1% BSA). Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.7% Imidazole
Constituents: 0.462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.632% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.638% Sodium chloride, 0.0165% Potassium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
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別名
- EL52
- epididymis luminal secretory protein 52
- Heat shock 86 kDa
see all -
機能
Molecular chaperone. Has ATPase activity. -
配列類似性
Belongs to the heat shock protein 90 family. -
翻訳後修飾
ISGylated. -
細胞内局在
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab80369 は 6 報の論文で使用されています。
- Pan X et al. Discovery of Quinacrine as a Potent Topo II and Hsp90 Dual-Target Inhibitor, Repurposing for Cancer Therapy. Molecules 27:N/A (2022). PubMed: 36080326
- Liu XY et al. Alkaloid Derivative (Z)-3β-Ethylamino-Pregn-17(20)-en Inhibits Triple-Negative Breast Cancer Metastasis and Angiogenesis by Targeting HSP90α. Molecules 27:N/A (2022). PubMed: 36296726
- Liu Z et al. Discovery of Small-Molecule Inhibitors of the HSP90-Calcineurin-NFAT Pathway against Glioblastoma. Cell Chem Biol 26:352-365.e7 (2019). PubMed: 30639261
- Li Z et al. Synthesis and biological evaluation of geldanamycin-ferulic acid conjugate as a potent Hsp90 inhibitor. RSC Adv 9:42509-42515 (2019). PubMed: 35542888
- Liang XH et al. Hsp90 protein interacts with phosphorothioate oligonucleotides containing hydrophobic 2'-modifications and enhances antisense activity. Nucleic Acids Res 44:3892-907 (2016). PubMed: 26945041
- Li Z et al. Discovery of novel 17-phenylethylaminegeldanamycin derivatives as potent Hsp90 inhibitors. Chem Biol Drug Des 85:181-8 (2015). PubMed: 24903735