Phosphate Assay Kit (Colorimetric) (ab65622)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts
- Sensitivity: 1 µM
製品の概要
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製品名
Phosphate Assay Kit (Colorimetric)
Phosphate キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media -
アッセイタイプ
Enzyme activity -
検出感度
> 1 µM -
検出範囲
0.001 mM - 1 mM -
全工程の試験時間
2h 00m -
製品の概要
Phosphate Assay Kit (Colorimetric) (ab65622) provides an easy, quick and simple method for measuring phosphate levels.
The phosphate assay protocol uses a proprietary formulation of malachite green and ammonium molybdate which forms a chromogenic complex with phosphate ion giving an intense absorption band around OD = 650nm.
Phosphate assay protocol summary:
- add reaction mix to sample and standard wells
- incubate for 30 min
- analyze with a microplate reader
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K410 Phosphate Colorimetric Assay Kit. K410-500 is the same size as the 500 test size of ab65622.
This assay can be used with biological fluids but also inorganic samples such as algal blooms and water from run-off areas of high fertilizer use.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at room temperature. Please refer to protocols. -
内容 500 tests Phosphate Reagent 1 x 15ml Phosphate Standard 1 x 500µl -
研究分野
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関連性
Phosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run-off from areas of high fertilizer use.
画像
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Immunoprecipitates of Flag-NT5C ectopically expressed in HEK293 cells were incubated with 5 mM of the indicated nucleotides. Phosphate release was measured using a malachite green colorimetric assay (ab65622) and expressed as a percent of total nucleotide. The experiment was performed in duplicate and repeated 3 times independently. Error bars are sem.
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Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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Phosphate measured in tissue lysates showing quantity (µmol) per mg of extracted protein.
Protein concentration for samples varied from 9 mg/mL to 14 mg/mL. Samples were diluted 400-4000 fold.
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Phosphate measured in cell culture lysates showing quantity (µmol) per mg of extracted protein.
Samples with the concentration of 1.3e7 cells/mL were used. Samples were diluted 400-4000 fold.
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Phosphate measured in biological fluids showing quantity (µmol) per mL of tested sample. Samples were diluted 100-1000 fold.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (81)
ab65622 は 81 報の論文で使用されています。
- Ponzetti M et al. Lipocalin 2 Influences Bone and Muscle Phenotype in the MDX Mouse Model of Duchenne Muscular Dystrophy. Int J Mol Sci 23:N/A (2022). PubMed: 35055145
- Liu X et al. Scaffold-Free Spheroids with Two-Dimensional Heteronano-Layers (2DHNL) Enabling Stem Cell and Osteogenic Factor Codelivery for Bone Repair. ACS Nano 16:2741-2755 (2022). PubMed: 35072461
- Sulaiman JE et al. Proteomics and Transcriptomics Uncover Key Processes for Elasnin Tolerance in Methicillin-Resistant Staphylococcus aureus. mSystems 7:e0139321 (2022). PubMed: 35076266
- Hou L et al. Modulation of myosin by cardiac myosin binding protein-C peptides improves cardiac contractility in ex-vivo experimental heart failure models. Sci Rep 12:4337 (2022). PubMed: 35288601
- Czaya B et al. Hyperphosphatemia increases inflammation to exacerbate anemia and skeletal muscle wasting independently of FGF23-FGFR4 signaling. Elife 11:N/A (2022). PubMed: 35302487