HRP Anti-COX IV 抗体 [mAbcam33985] - Mitochondrial Marker (ab62164)
Key features and details
- HRP Mouse monoclonal [mAbcam33985] to COX IV - Mitochondrial Marker
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Conjugation: HRP
- Isotype: IgG
Related conjugates and formulations
製品の概要
-
製品名
HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker
COX IV 一次抗体 製品一覧 -
製品の詳細
HRP Mouse monoclonal [mAbcam33985] to COX IV - Mitochondrial Marker -
由来種
Mouse -
標識
HRP -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Xenopus laevis, Chimpanzee, Zebrafish -
免疫原
Synthetic peptide corresponding to Human COX IV aa 150 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab16381) -
ポジティブ・コントロール
- WB: HeLa, HEK293, A431, Jurkat and HepG2 whole cell lysates and mouse skeletal muscle tissue lysate. IHC-P: Normal human colon tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
-
精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
mAbcam33985 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Recombinant Protein
-
Related Products
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62164の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/5000. Predicted molecular weight: 15 kDa.
|
|
IHC-P |
1/100.
|
特記事項 |
---|
WB
1/5000. Predicted molecular weight: 15 kDa. |
IHC-P
1/100. |
ターゲット情報
-
機能
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the cytochrome c oxidase IV family. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 454391 Chimpanzee
- Entrez Gene: 1327 Human
- Entrez Gene: 12857 Mouse
- Entrez Gene: 29445 Rat
- Entrez Gene: 326975 Zebrafish
- Omim: 123864 Human
- SwissProt: O46577 Chimpanzee
- SwissProt: P00423 Cow
see all -
別名
- AL024441 antibody
- COX 4 antibody
- COX IV 1 antibody
see all
画像
-
IHC image of COX IV staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab62164 at 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
-
All lanes : HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164) at 1/5000 dilution
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab62164 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
-
All lanes : HRP Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab62164) at 1/4000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (5)
ab62164 は 5 報の論文で使用されています。
- Lobo-Jarne T et al. Multiple pathways coordinate assembly of human mitochondrial complex IV and stabilization of respiratory supercomplexes. EMBO J N/A:e103912 (2020). PubMed: 32511785
- Lowe MM et al. Regulatory T cells use arginase 2 to enhance their metabolic fitness in tissues. JCI Insight 4:N/A (2019). PubMed: 31852848
- Serricchio M et al. Cardiolipin synthesizing enzymes form a complex that interacts with cardiolipin-dependent membrane organizing proteins. Biochim Biophys Acta 1863:447-457 (2018). PubMed: 29343430
- Di K et al. Mitochondrial Lon is over-expressed in high-grade gliomas, and mediates hypoxic adaptation: potential role of Lon as a therapeutic target in glioma. Oncotarget 7:77457-77467 (2016). WB ; Human . PubMed: 27764809
- Powell KA et al. Mitochondrial localization and ocular expression of mutant Opa3 in a mouse model of 3-methylglutaconicaciduria type III. Invest Ophthalmol Vis Sci 52:4369-80 (2011). PubMed: 21613372