Recombinant human AKT2 protein (ab60322)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
製品の詳細
-
製品名
Recombinant human AKT2 protein
AKT2 タンパク質・ペプチド 製品一覧 -
精製度
> 90 % SDS-PAGE.
Purity: >90% as determined by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human
-
関連製品
-
Related Products
-
Substrate reagent
特性
Our Abpromise guarantee covers the use of ab60322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
SDS-PAGE
Functional Studies
-
製品の状態
Liquid -
備考
ab204851 (AKT peptide) can be utilized as a substrate for assessing kinase activity
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
別名
- AKT
- Akt2
- AKT2_HUMAN
see all -
機能
General protein kinase capable of phosphorylating several known proteins. -
組織特異性
Expressed in all human cell types so far analyzed. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 PH domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylation on Thr-309 and Ser-474 is required for full activity.
Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. TRAF6-induced 'Lys-63'-linked AKT2 ubiquitination. When fully phosphorylated and translocated into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its degradation by the proteasome. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab60322 は論文での使用が確認できていません。