Recombinant Human IKB alpha protein (ab59981)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% Densitometry
- Tags: GST tag N-Terminus
- Suitable for: WB, SDS-PAGE
製品の詳細
-
製品名
Recombinant Human IKB alpha protein
IKB alpha タンパク質・ペプチド 製品一覧 -
精製度
> 80 % Densitometry. -
発現系
Escherichia coli -
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
予測される分子量
60 kDa including tags -
領域
1 to 317 -
タグ
GST tag N-Terminus
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab59981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Western blot
SDS-PAGE
-
製品の状態
Liquid -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol, 0.87% Sodium chloride
関連情報
-
別名
- I kappa B alpha
- I-kappa-B-alpha
- IkappaBalpha
see all -
機能
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. -
関連疾患
Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant -
配列類似性
Belongs to the NF-kappa-B inhibitor family.
Contains 5 ANK repeats. -
翻訳後修飾
Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation. -
細胞内局在
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. - Information by UniProt
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab59981 は 2 報の論文で使用されています。
- Lanucara F et al. Dynamic phosphorylation of RelA on Ser42 and Ser45 in response to TNFa stimulation regulates DNA binding and transcription. Open Biol 6:N/A (2016). PubMed: 27466442
- Dong T et al. Ainsliadimer A selectively inhibits IKKa/ß by covalently binding a conserved cysteine. Nat Commun 6:6522 (2015). PubMed: 25813672