Anti-CCR7 抗体 [4B12] (ab52602)
Key features and details
- Rat monoclonal [4B12] to CCR7
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
製品の概要
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製品名
Anti-CCR7 antibody [4B12]
CCR7 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [4B12] to CCR7 -
由来種
Rat -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow Cyt: Mouse splenocytes.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
4B12 -
アイソタイプ
IgG2a -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab52602の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
Use a concentration of 5 - 10 µg/ml.
ab18541 - Rat IgG2b monoclonal, is suitable for use as an isotype control with this antibody. |
特記事項 |
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Flow Cyt
Use a concentration of 5 - 10 µg/ml. ab18541 - Rat IgG2b monoclonal, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. -
組織特異性
Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 12775 Mouse
- SwissProt: P47774 Mouse
- Unigene: 2932 Mouse
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別名
- BLR 2 antibody
- BLR2 antibody
- C C chemokine receptor type 7 antibody
see all
画像
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C57BL6 mouse splenocytes were incubated with Rat IgG2b, kappa monoclonal [RTK4530] (ab18541) (left) or anti-CCR7 [4B12] antibody (ab52602) (right) at 10ug/ml for 30 min at 4+°C. The secondary antibody Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157) was used at 1/2000 dilution for 30 min at 4+°C. The cells were simultaneously stained with anti-CD3ε PerCP (10ug/ml) (ab190299).
Acquisition of >30,000 total events were collected. Gating strategy - live lymphocytes
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab52602 は 2 報の論文で使用されています。
- Ritter U et al. Analysis of the CCR7 expression on murine bone marrow-derived and spleen dendritic cells. J Leukoc Biol 76:472-6 (2004). PubMed: 15197229
- Ohl L et al. CCR7 governs skin dendritic cell migration under inflammatory and steady-state conditions. Immunity 21:279-88 (2004). PubMed: 15308107