Anti-DARPP32 抗体 [EP720Y] (ab40801)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP720Y] to DARPP32
- Suitable for: ICC/IF, WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-DARPP32 antibody [EP720Y]
DARPP32 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP720Y] to DARPP32 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-P, IPmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Pig -
免疫原
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ポジティブ・コントロール
- IHC-P: Human breast adenocarcinoma tissue and colon tissue. Mouse and rat cerebral cortex tissue. ICC/IF: Mouse brain tissue. WB: Rat hippocampus and brain tissue lysate. Mouse cerebral cortex tissue lysate. Human fetal brain lysate. IP: Rat brain whole cell lysate. IHC-Wm: Mouse brain tissue. IHC-Fr: Pig brain tissue.
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特記事項
Protein phosphatase 1 regulatory subunit 1B (PPP1R1B) is a protein that in humans is encoded by the PPP1R1B gene. This gene, also known as DARPP-32, has a role as a dopamine- and cyclic AMP-regulated phosphoprotein. As such PPP1R1B affects dopamine glutamate and adenosine; and potentially has a role in conditions such as schizophrenia and Parkinson's disease.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP720Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40801の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (2) |
1/50 - 1/100.
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WB | (3) |
1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
For unpurified use at 1/5000 - 1/50 000. |
IHC-P |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/20.
For unpurified use at 1/50. |
特記事項 |
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ICC/IF
1/50 - 1/100. |
WB
1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). For unpurified use at 1/5000 - 1/50 000. |
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/20. For unpurified use at 1/50. |
ターゲット情報
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機能
Inhibitor of protein-phosphatase 1. -
配列類似性
Belongs to the protein phosphatase inhibitor 1 family. -
翻訳後修飾
Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 84152 Human
- Entrez Gene: 19049 Mouse
- Entrez Gene: 360616 Rat
- Omim: 604399 Human
- SwissProt: Q9UD71 Human
- SwissProt: Q60829 Mouse
- SwissProt: Q6J4I0 Rat
- Unigene: 286192 Human
see all -
別名
- DARPP 32 antibody
- DARPP-32 antibody
- Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded human colon sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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All lanes : Anti-DARPP32 antibody [EP720Y] (ab40801) at 1/50000 dilution (purified)
Lane 1 : Rat hippocampus tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Mouse cerebral cortex tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDaBlocking/Diluting buffer 5% NFDM/TBST.
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Immunocytochemistry/Immunofluorescence analysis of mouse brain tissue labelling DARPP32 with purified ab40801 at 1/100 (3.4 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was performed with Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody.
Secondary Only Control: PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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ab40801 at 1/20 immunoprecipitating DARPP32 in rat brain whole cell lysate observed at 32 KDa (lanes 1 and 2).
Lane 1: Rat brain whole cell lysate 10μg.
Lane 2: ab40801 IP in rat brain whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab40801 in rat brain whole cell lysate.
For western blotting, ab131366 VeriBlot for IP (HRP) was used for detection (1/1000).
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded mouse cerebral cortex sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
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Anti-DARPP32 antibody [EP720Y] (ab40801) at 1/1000 dilution (purified) + Human fetal brain lysate at 15 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa
Blocking/Diluting buffer 5% NFDM/TBST -
Immunohistochemical analysis of human breast adenocarcinoma sections labelling DARPP32 with unpurified ab40801 at a dilution of 1/50.
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Anti-DARPP32 antibody [EP720Y] (ab40801) at 1/50000 dilution (unpurified) + Rat brain lysate at 10 µg
Predicted band size: 32 kDa
Observed band size: 32 kDa -
Immunohistochemical analysis of paraffin-embedded rat cerebral cortex sections labelling DARPP32 with purified ab40801 at dilution of 1/50. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was performed using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (96)
ab40801 は 96 報の論文で使用されています。
- Morelli KH et al. An RNA-targeting CRISPR-Cas13d system alleviates disease-related phenotypes in Huntington's disease models. Nat Neurosci 26:27-38 (2023). PubMed: 36510111
- Gangwani MR et al. Neuronal and astrocytic contributions to Huntington's disease dissected with zinc finger protein transcriptional repressors. Cell Rep 42:111953 (2023). PubMed: 36640336
- Miura Y et al. Engineering brain assembloids to interrogate human neural circuits. Nat Protoc 17:15-35 (2022). PubMed: 34992269
- Palminha NM et al. Defective repair of topoisomerase I induced chromosomal damage in Huntington's disease. Cell Mol Life Sci 79:160 (2022). PubMed: 35224690
- Song H et al. Suppression of toxicity of the mutant huntingtin protein by its interacting compound, desonide. Proc Natl Acad Sci U S A 119:e2114303119 (2022). PubMed: 35238684