Anti-Vimentin 抗体 (ab39376)
Key features and details
- Chicken polyclonal to Vimentin
- Suitable for: ICC/IF
- Reacts with: Mouse
- Isotype: IgY
製品の概要
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製品名
Anti-Vimentin antibody
Vimentin 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to Vimentin -
由来種
Chicken -
アプリケーション
適用あり: ICC/IFmore details -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide corresponding to a region of the Vimentin gene product shared between the mouse (NP_035831, NCBI) and human (NP_003371, NCBI) gene products.
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ポジティブ・コントロール
- ICC/IF: Mouse brain
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS
(10mM isotonic 0.9% w/v), pH 7.2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab39376の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | (5) |
1/100 - 1/200.
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特記事項 |
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ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
組織特異性
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
関連疾患
Cataract 30 -
配列類似性
Belongs to the intermediate filament family. -
ドメイン
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
翻訳後修飾
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 22352 Mouse
- SwissProt: P20152 Mouse
- Unigene: 268000 Mouse
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製品の状態
Vimentin is found in connective tissue and in the cytoskeleton. -
別名
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab39376 は 8 報の論文で使用されています。
- Treviño-Villarreal JH et al. Down-Regulation of a Profibrotic Transforming Growth Factor-β1/Cellular Communication Network Factor 2/Matrix Metalloprotease 9 Axis by Triamcinolone Improves Idiopathic Subglottic Stenosis. Am J Pathol 191:1412-1430 (2021). PubMed: 34111429
- Sinkey RG et al. Thrombin-Induced Decidual Colony-Stimulating Factor-2 Promotes Abruption-Related Preterm Birth by Weakening Fetal Membranes. Am J Pathol 190:388-399 (2020). PubMed: 31955792
- Mong EF et al. Chromosome 19 microRNA cluster enhances cell reprogramming by inhibiting epithelial-to-mesenchymal transition. Sci Rep 10:3029 (2020). PubMed: 32080251
- Su N et al. Occurrence of Transmembrane Protein 119 in the Retina is Not Restricted to the Microglia: An Immunohistochemical Study. Transl Vis Sci Technol 8:29 (2019). PubMed: 31853425
- Guzeloglu-Kayisli O et al. Mechanisms of chorioamnionitis-associated preterm birth: interleukin-1β inhibits progesterone receptor expression in decidual cells. J Pathol 237:423-34 (2015). PubMed: 26175191
- Mulholland DJ et al. Pten loss and RAS/MAPK activation cooperate to promote EMT and metastasis initiated from prostate cancer stem/progenitor cells. Cancer Res 72:1878-89 (2012). IHC . PubMed: 22350410
- Schilte C et al. Type I IFN controls chikungunya virus via its action on nonhematopoietic cells. J Exp Med 207:429-42 (2010). IHC-Fr ; Mouse . PubMed: 20123960
- Lawson DA et al. Basal epithelial stem cells are efficient targets for prostate cancer initiation. Proc Natl Acad Sci U S A 107:2610-5 (2010). ICC/IF ; Mouse . PubMed: 20133806