Recombinant human CNTF protein (Active) (ab283456)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% HPLC
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, Cell Culture, SDS-PAGE, HPLC, MS
製品の詳細
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製品名
Recombinant human CNTF protein (Active)
CNTF タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by dose dependent proliferation of TF-1 cells. ED50 is ≤4.914 µg/ml, corresponding to a specific activity of 2.04 x 105 units/mg.
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精製度
>= 95 % HPLC. -
エンドトキシン・レベル
<=0.005 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Human -
配列
AFTEHSPLTPHRRDLCSRSIWLARKIRSDLTALTESYVKHQGLNKNINLD SADGMPVASTDQWSELTEAERLQENLQAYRTFHVLLARLLEDQQVHFTPT EGDFHQAIHTLLLQVAAFAYQIEELMILLEYKIPRNEADGMPINVGDGGL FEKKLWGLKVLQELSQWTVRSIHDLRFISSHQTGIPARGSHYIANNKKM -
予測される分子量
23 kDa -
実際の分子量
23 kDa -
分子量情報
Predicted MW is 22856.99 Da (+/- 10 Da by ESI-TOF). Observed MW is 22858.40 Da -
領域
2 to 200 -
配列の追加情報
N-terminal glycine
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特性
Our Abpromise guarantee covers the use of ab283456 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
Functional Studies
Cell Culture
SDS-PAGE
HPLC
Mass Spectrometry
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製品の状態
Lyophilized -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Store lyophilized form at room temperature. Reconstitute in PBS, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- Ciliary neurotrophic factor
- CNTF
- CNTF_HUMAN
- HCNTF
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機能
CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. -
組織特異性
Nervous system. -
配列類似性
Belongs to the CNTF family. -
細胞内局在
Cytoplasm. - Information by UniProt
画像
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SDS-page analysis of ab283456
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Mass determination by ESI-TOF.
Predicted MW is 22856.99 Da (+/- 10 Da by ESI-TOF). Observed MW is 22858.40 Da
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HPLC analysis of ab283456
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Fully biologically active determined by dose dependent proliferation of TF-1 cells. ED50 is ≤4.914 µg/ml, corresponding to a specific activity of 2.04 x 105 units/mg.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab283456 は論文での使用が確認できていません。