Human PD-L1 ELISA Kit [28-8] (ab277712)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.75 pg/ml
- Range: 7.81 pg/ml - 500 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human PD-L1 ELISA Kit [28-8]
PD-L1 キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Supernatant 8 2.7% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Supernatant 3 4.3% -
サンプルの種類
Cell culture supernatant, Urine, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
3.75 pg/ml -
検出範囲
7.81 pg/ml - 500 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 93 82% - 100% Urine 93 86% - 97% Serum 96 94% - 97% Cell culture extracts 102 99% - 106% Hep Plasma 101 90% - 111% EDTA Plasma 90 89% - 92% Cit plasma 90 87% - 95% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Human -
製品の概要
Human PD-L1 ELISA kit [28-8] (ab277712) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PD-L1 protein in human serum, plasma, cell culture supernatant, urine, and cell extract samples. It uses our proprietary SimpleStep ELISA® technology and our recombinant PD-L1 antibody clone [28-8]. Quantitate Human PD-L1 with 3.75 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
PD-L1 (also known as CD274 or B7-H1) is a membrane bound glycoprotein involved in regulation of the immune system. PD-L1 is expressed on a variety of inflammatory-activated cells as well as some carcinomas and in melanoma. PD-L1 binds to PD-1 and CD80, where it can suppress T cell activation and proliferation as well as induce apoptosis. Levels of PD-L1 are increased in the plasma of cancer patients as well as in cerebrospinal fluid of gliomas. PD-L1 can bind PD-1 in order to regulate T cell apoptosis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 1 x 96 tests 1 x 96 tests 10X Human PD-L1 [28-8] Detector Antibody 1 x 600µl 1 x 600µl 1 x 600µl 10X Human PD-L1 [YCA-R23939-25] Capture Antibody 1 x 600µl 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml 1 x 10ml 1 x 10ml Antibody Diluent CPI2 1 x 6ml 1 x 6ml 1 x 6ml Human PD-L1 [28-8] Lyophilized Recombinant Protein 2 vials 2 vials 2 vials Plate Seals 1 unit 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 12ml 1 x 12ml 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 unit 1 unit Stop Solution 1 x 12ml 1 x 12ml 1 x 12ml TMB Development Solution 1 x 12ml 1 x 12ml 1 x 12ml -
研究分野
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機能
Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production. -
組織特異性
Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes. -
配列類似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Cell membrane and Endomembrane system. - Information by UniProt
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別名
- B7 H
- B7 H1
- B7 homolog 1
see all -
参照データベース
- Entrez Gene: 29126 Human
- Omim: 605402 Human
- SwissProt: Q9NZQ7 Human
- Unigene: 521989 Human
関連製品
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Alternative Versions
画像
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Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Undiluted samples are as follows: serum 95% and PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L1 concentration was determined to be 111.81 pg/mL in serum and 361.74 pg/mL in stimulated PBMC supernatant.
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The concentrations of PD-L1 were measured in duplicate and interpolated from the PD-L1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L1 concentration was determined to be 393.85 pg/mL in HDLM-2 extract.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PD-L1 concentration was determined to be 99.45 pg/mL with a range of 59.76 – 168.01 pg/mL.
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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To learn more about the advantages of recombinant antibodies see here.
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To learn more about the advantages of SimpleStep ELISA® kits see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab277712 は論文での使用が確認できていません。