Biotin Anti-Macrophage Inflammatory Protein 3 alpha 抗体 (ab271284)
Key features and details
- Biotin Rabbit polyclonal to Macrophage Inflammatory Protein 3 alpha
- Suitable for: WB, Sandwich ELISA
- Reacts with: Recombinant fragment
- Conjugation: Biotin
- Isotype: IgG
製品の概要
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製品名
Biotin Anti-Macrophage Inflammatory Protein 3 alpha antibody
Macrophage Inflammatory Protein 3 alpha 一次抗体 製品一覧 -
製品の詳細
Biotin Rabbit polyclonal to Macrophage Inflammatory Protein 3 alpha -
由来種
Rabbit -
標識
Biotin -
アプリケーション
適用あり: WB, Sandwich ELISAmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human Macrophage Inflammatory Protein 3 alpha aa 1 to the C-terminus. Mature chain. E. coli derived.
Database link: P78556 -
ポジティブ・コントロール
- WB: Recombinant human Macrophage Inflammatory Protein 3 alpha.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute in sterile PBS with 0.1% BSA to 0.1 - 1.0 mg/ml. -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab271284の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 10 kDa.
When used in conjunction with compatible secondary reagents, the detection limit for recombinant human Macrophage Inflammatory Protein 3 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|
Sandwich ELISA |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 10 kDa. When used in conjunction with compatible secondary reagents, the detection limit for recombinant human Macrophage Inflammatory Protein 3 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Sandwich ELISA
Use at an assay dependent concentration. |
ターゲット情報
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機能
Chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. Inhibits proliferation of myeloid progenitors in colony formation assays. May be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. Possesses antibacterial activity E.coli ATCC 25922 and S.aureus ATCC 29213. -
組織特異性
Expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels seen in thymus, prostate, testis, small intestine and colon. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
翻訳後修飾
C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Beta chemokine exodus 1 antibody
- Beta-chemokine exodus-1 antibody
- C C motif chemokine ligand 20 antibody
see all
画像
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Lane 1: Marker.
Lane 2: Recombinant hMIP3 alpha at 250ng/lane.
Lane 3: Recombinant hMIP3 alpha at 125ng/lane.
Lane 4: Recombinant hMIP3 alpha at 62.5ng/lane.
Lane 5: Recombinant hMIP3 alpha at 31.25ng/lane.
Lane 6: Recombinant hMIP3 alpha at 15.625ng/lane.
Lane 7: Recombinant hMIP3 alpha at 7.8ng/lane.
Lane 8: Recombinant hMIP3 alpha at 3.9ng/lane.
Lane 9: Recombinant hMIP3 alpha at 1.95ng/lane.
Lane 10: Recombinant hMIP3 alpha at 0.975ng/lane.
Lane 11: Recombinant hMIP3 alpha at 0.4875ng/lane.
Lane 12: Recombinant hMIP3 alpha at 0.24ng/lane. -
Human Macrophage Inflammatory Protein 3 alpha was detected by sandwich ELISA (using 100μl/well) using a concentration of 0.25-1.0 μg/ml of ab271284. This antibody in conjunction with an appropriate capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant Macrophage Inflammatory Protein 3 alpha protein.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab271284 は論文での使用が確認できていません。