Protein A Gold Conjugate (40nm, 10 OD) (ab270695)
製品の概要
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製品名
Protein A Gold Conjugate (40nm, 10 OD) -
特記事項
Protein A Gold Conjugate (40nm, 10 OD) (ab270695) is manufactured using our Gold Nanoparticles which have a protective coating that can withstand the most extreme conditions (e.g. 2.5 M NaOH at 70°C for >1 hour). The irreversible covalent attachment does not allow the desorption of the Protein A, which is a major problem associated with gold conjugates when developed using passive adsorption techniques.
To exchange the conjugate into a specific buffer for your assay or test, centrifuge the conjugate in a microfuge at 9,000 x g for 6 minutes. Carefully remove the supernatant and add your preferred buffer. It is important to avoid substances that have a very high affinity for gold (e.g. thiols).
The maximum absorbance for Protein A Gold Conjugate (40nm, 10 OD) is at 530 nm. To determine the effective concentration of the conjugate obtained we advise to measure the Absmax of light using an UV-vis spectrophotometer after diluting your sample to an appropriate range for your piece of equipment (e.g. if a 40 nm gold conjugate is at 10 OD and is diluted 1:10 the Abs530nm for a 1 cm light path is expected to be around 1 AU).
Before using Protein A Gold Conjugate (40nm, 10 OD) to detect a specific antibody we recommend that you check the affinity of Protein A for that antibody and subtype (see table below). Rabbit and human IgGs, for example, are good candidates because they bind to Protein A with high affinity.
Species
IgG
Binding Strength
Rabbit
IgG
High
Human
IgG
High
Pig
IgG
High
Mouse
IgG1
Low/Medium
Mouse
IgG2a
High
Mouse
IgG2b
High
Mouse
IgG3
Low/Medium
Goat
IgG
Low
Sheep
IgG
Low
Rat
IgG
Low
Protein A Gold Conjugate (40nm, 10 OD) is tested as a detector tool in Lateral Flow Assay but can also be used in electron and light microscopy, immunoblotting, and dot-blot. As Protein A (40nm Gold) affinity for antibodies can vary, a titration of the specific antibody used is recommended in order to obtain the optimal performance. We recommend performing the incubation of Protein A Gold Conjugate (40nm, 10 OD) with the antibody in a buffer that has a pH close to 8, for at least 10-15 minutes. After this stage do not centrifuge the gold complex.
This product is manufactured by Expedeon, an Abcam company. It was previously called 40nm InnovaCoat® GOLD (10 OD) Protein A Conjugate. Expedeon product code 222-0200 is the same as the 200 µl size of this product and code 222-1000 is the same as the 1 mL size.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at Room Temperature. Store at +4°C. -
バッファー
pH: 7
Constituents: Tris buffered saline, 0.1% Tween -
Concentration information loading...
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab270695 は論文での使用が確認できていません。