Mouse LBP ELISA Kit (LPS Binding Protein) (ab269542)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.34 pg/ml
- Range: 50 pg/ml - 3200 pg/ml
- Sample type: Cell culture media, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
製品の概要
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製品名
Mouse LBP ELISA Kit (LPS Binding Protein)
LBP キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Serum 8 7.5% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Serum 3 10.1% -
サンプルの種類
Serum, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
8.34 pg/ml -
検出範囲
50 pg/ml - 3200 pg/ml -
添加回収試験
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Serum 84 83% - 85% Cell culture media 99 92% - 103% Hep Plasma 100 95% - 104% EDTA Plasma 97 96% - 99% Cit plasma 103 101% - 105% -
全工程の試験時間
1h 30m -
ステップ
One step assay -
種交差性
交差種: Mouse -
製品の概要
Mouse LBP ELISA Kit (LPS Binding Protein) (ab269542) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of LBP (LPS Binding Protein) protein in cell culture media, edta plasma, hep plasma, serum, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse LBP (LPS Binding Protein) with 8.34 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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特記事項
Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.
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試験プラットフォーム
Pre-coated microplate (12 x 8 well strips)
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 x 96 tests 1 x 96 tests 10X Mouse LBP (LPS Binding Protein) Capture Antibody 1 x 600µl 1 x 600µl 10X Mouse LBP (LPS Binding Protein) Detector Antibody 1 x 600µl 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 1 x 20ml Antibody Diluent CPR2 1 x 6ml 1 x 6ml Mouse LBP (LPS Binding Protein) Lyophilized Recombinant Protein 2 vials 2 vials Plate Seals 1 unit 1 unit Sample Diluent NS (ab193972) 1 x 50ml 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit 1 unit Stop Solution 1 x 12ml 1 x 12ml TMB Development Solution 1 x 12ml 1 x 12ml -
研究分野
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機能
Binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria. The LBP/LPS complex seems to interact with the CD14 receptor. -
配列類似性
Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- BPI fold containing family D, member 2
- Bpifd2
- LBP
see all -
参照データベース
- Entrez Gene: 16803 Mouse
- SwissProt: Q61805 Mouse
- Unigene: 218846 Mouse
関連製品
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Alternative Versions
画像
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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The LBP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of LBP were measured in duplicates, interpolated from the LBP standard curves and corrected for sample dilution. Undiluted samples referred to suggested minimum dilution as follows: serum 1: 1,000, plasma (citrate) 1: 2,000, plasma (heparin) 1: 1,000, and plasma (EDTA) 1: 2,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean LBP concentration was determined to be 1.06 µg/mL in serum, 2.37 µg/mL in plasma (citrate), 1.53 µg/mL in plasma (heparin), and 2.12 µg/mL in plasma (EDTA).
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To learn more about the advantages of recombinant antibodies see here.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab269542 は 6 報の論文で使用されています。
- Zhao Y et al. Berberine ameliorates aGVHD by gut microbiota remodelling, TLR4 signalling suppression and colonic barrier repairment for NLRP3 inflammasome inhibition. J Cell Mol Med 26:1060-1070 (2022). PubMed: 34984827
- Wolstenholme JT et al. Reduced alcohol preference and intake after fecal transplant in patients with alcohol use disorder is transmissible to germ-free mice. Nat Commun 13:6198 (2022). PubMed: 36261423
- Yuan X et al. Functional and metabolic alterations of gut microbiota in children with new-onset type 1 diabetes. Nat Commun 13:6356 (2022). PubMed: 36289225
- Yang W et al. Translocation and Dissemination of Gut Bacteria after Severe Traumatic Brain Injury. Microorganisms 10:N/A (2022). PubMed: 36296362
- Wang K et al. The negative effect of Akkermansia muciniphila-mediated post-antibiotic reconstitution of the gut microbiota on the development of colitis-associated colorectal cancer in mice. Front Microbiol 13:932047 (2022). PubMed: 36312913
- Wang RR et al. Structural and Functional Modulation of Gut Microbiota by Jiangzhi Granules during the Amelioration of Nonalcoholic Fatty Liver Disease. Oxid Med Cell Longev 2021:2234695 (2021). PubMed: 34966475