Anti-Phosphotyrosine 抗体 [Py72] (ab269515)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [Py72] to Phosphotyrosine
- Suitable for: WB, IP
- Reacts with: Species independent
Related conjugates and formulations
製品の概要
-
製品名
Anti-Phosphotyrosine antibody [Py72]
Phosphotyrosine 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [Py72] to Phosphotyrosine -
由来種
Mouse -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Species independent -
免疫原
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Pervanadate treated NIH/3T3, Daudi and MCF7 whole cell lysates. IP: Pervanadate treated NIH/3T3 and MCF7 whole cell lysates.
-
特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
Py72 -
アイソタイプ
IgG1 -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab269515の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 0.196 µg/ml.
|
|
IP |
Use a concentration of 32.667 µg/ml.
|
特記事項 |
---|
WB
Use a concentration of 0.196 µg/ml. |
IP
Use a concentration of 32.667 µg/ml. |
ターゲット情報
-
関連性
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
画像
-
All lanes : Anti-Phosphotyrosine antibody [Py72] (ab269515) at 0.196 µg/ml
Lane 1 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 2 : NIH/3T3 treated with 10mM pervanadate for 20 minutes, whole cell lysates
Lane 3 : Daudi (human Burkitt's lymphoma lymphoblast), whole cell lysate
Lane 4 : Daudi treated with 10mM pervanadate for 30 minutes, whole cell lysates
Lane 5 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 6 : MCF7 treated with 1mM pervanadate for 20 minutes, whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilutionMultiple bands represent phosph-tyrosine containing proteins detected by ab269515.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1-4: 3.25 secs; Lanes 5-6: 3 mins.
-
Phosphotyrosine was immunoprecipitated from MCF7 (human breast adenocarcinoma epithelial cell), treated with 1mM pervanadate for 20 minutes, whole cell lysates, with ab269515 at 1/30 dilution (2µg in 0.35mg lysates, (32.667µg/ml)). Western blot was performed on the immunoprecipitate using ab269515 at 1/1000 dilution (0.196µg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: MCF7 treated with 1mM pervanadate for 20 mins, whole cell lysates, 10µg.
Lane 2: ab269515 IP in MCF7 treated with 1mM pervanadate for 20 mins, whole cell lysates.
Lane 3: Mouse monoclonal IgG1 (ab18443) instead of ab269515 in MCF7 treated with 1mM pervanadate for 20 mins, whole cell lysates.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Multiple bands represent phosph-tyrosine containing proteins precipitated and detected by ab269515.
-
Phosphotyrosine was immunoprecipitated from NIH/3T3 (Mouse embyro fibroblast cells), treated with 10mM pervanadate for 20 minutes, whole cell lysates, with ab269515 at 1/30 dilution (2µg in 0.35mg lysates, (32.667µg/ml)). Western blot was performed on the immunoprecipitate using ab269515 at 1/1000 dilution (0.196µg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: NIH/3T3 treated with 10mM pervanadate for 20 mins, whole cell lysates, 10µg.
Lane 2: ab269515 IP in NIH/3T3 treated with 10mM pervanadate for 20 mins, whole cell lysates.
Lane 3: Mouse monoclonal IgG1 (ab18443) instead of ab269515 in NIH/3T3 treated with 10mM pervanadate for 20 mins, whole cell lysates.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
Multiple bands represent phosph-tyrosine containing proteins precipitated and detected by ab269515.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab269515 は論文での使用が確認できていません。