Anti-Wnt16 抗体 [EPR22889-129] (ab269278)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22889-129] to Wnt16
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Wnt16 antibody [EPR22889-129]
Wnt16 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22889-129] to Wnt16 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: IMR-90 and HEK-293 whole cell lysates. IP: HEK-293 whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22889-129 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab269278の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa).
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IP |
1/30.
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特記事項 |
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WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 41 kDa). |
IP
1/30. |
ターゲット情報
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機能
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. -
組織特異性
Isoform Wnt-16b is expressed in peripheral lymphoid organs such as spleen, appendix, and lymph nodes, in kidney but not in bone marrow. Isoform Wnt-16a is expressed at significant levels only in the pancreas. -
配列類似性
Belongs to the Wnt family. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 51384 Human
- Omim: 606267 Human
- SwissProt: Q9UBV4 Human
- Unigene: 272375 Human
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別名
- Protein Wnt 16 precursor antibody
- Protein Wnt-16 antibody
- Wingless type MMTV integration site family member 16 antibody
see all
画像
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All lanes : Anti-Wnt16 antibody [EPR22889-129] (ab269278) at 1/1000 dilution
Lane 1 : IMR-90 (human lung fibroblast) whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell
Lane 3 : U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 41 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe expression profile observed is consistent with what has been described in the literature, the band of 45 KDa represents the full length Wnt16, the band of 32 KDa represents the degraded form of Wnt16. (PMID:26254351, 17200136).
Negative control: U-2 OS (PMID: 25999350).
This blot was developed using a higher sensitivity ECL substrate.Blocking/Diluting Buffer: 5% NFDM/TBST
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Wnt16 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate using ab269278 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab269278 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used as the secondary anyibody at 1/5000 dilution.
Lane 1: HEK-293 whole cell lysate (input)
Lane 2: ab269278 IP in HEK-293 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab269278 in HEK-293 whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 3 mintues
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab269278 は論文での使用が確認できていません。