Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) 抗体 [MsMab-1] (ab264055)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [MsMab-1] to IDH1 (mutated R132G) + IDH2 (mutated R172G)
- Suitable for: WB, ELISA
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] -
製品の詳細
Mouse monoclonal [MsMab-1] to IDH1 (mutated R132G) + IDH2 (mutated R172G) -
由来種
Mouse -
特異性
The antibody also showed the cross reactivity with other mutants of IDH1 and IDH2.
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アプリケーション
適用あり: WB, ELISAmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: His-tagged human IDH1 mutated R132G (aa1-414), His-tagged human IDH2 mutated R172G (aa40-452), His-tagged human IDH1 mutated R132H (aa1-414), His-tagged human IDH1 mutated R132S (aa1-414), His-tagged human IDH2 mutated R172S (aa40-452), recombinant protein.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
MsMab-1 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab264055の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000.
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ELISA |
Use a concentration of 0.25 µg/ml.
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特記事項 |
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WB
1/1000. |
ELISA
Use a concentration of 0.25 µg/ml. |
ターゲット情報
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細胞内局在
IDH1: Cytoplasm. Peroxisome. IDH2: Mitochondrion. -
参照データベース
- Entrez Gene: 3417 Human
- Entrez Gene: 3418 Human
- Omim: 147650 Human
- Omim: 147700 Human
- SwissProt: O75874 Human
- SwissProt: P48735 Human
- Unigene: 593422 Human
- Unigene: 596461 Human
画像
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All lanes : Anti-IDH1 (mutated R132G) + IDH2 (mutated R172G) antibody [MsMab-1] (ab264055) at 1/1000 dilution
Lane 1 : His-tagged human IDH1 (aa1-414) recombinant protein
Lane 2 : His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein
Lane 3 : His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein
Lane 4 : His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein
Lane 5 : His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein
Lane 6 : His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein
Lane 7 : His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein
Lane 8 : His-tagged human IDH2 (aa40-452) recombinant protein
Lane 9 : His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein
Lane 10 : His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein
Lane 11 : His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein
Lane 12 : His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein
Lane 13 : His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein
Lane 14 : His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Observed band size: 46 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
The loading samples are E.coil extracts containing recombinant protein respectively.
Exposure time: 10 seconds
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Antigen: Human IDH1, Human IDH1(R132H), Human IDH1(R132G), Human IDH1(R132L), Human IDH1(R132S), Human IDH1(R132V), Human IDH1(R132C)).
Antigen concentration: 50 ng/ml.
ab264055 used at 0 - 1000 ng/ml.
Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L)secondary antibody was used at 1:1000 dilution.
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Antigen: Human IDH2, Human IDH2(R172G), Human IDH2(R172M), Human IDH2(R172S), Human IDH2(R172W), Human IDH2(R172K), Human IDH2(R140Q).
Antigen concentration: 50 ng/ml.
ab264055 used at 0 - 1000 ng/ml.
Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L)secondary antibody was used at 1:1000 dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab264055 は 2 報の論文で使用されています。
- Kitago Y et al. Structural basis for multi-specific peptide recognition by the anti-IDH1/2 monoclonal antibody, MsMab-1. Biochem Biophys Res Commun 478:1274-9 (2016). PubMed: 27553275
- Kato Kaneko M et al. Establishment of a multi-specific monoclonal antibody MsMab-1 recognizing both IDH1 and IDH2 mutations. Tohoku J Exp Med 230:103-9 (2013). PubMed: 23782684