Anti-IDH2 (mutated R172K) 抗体 [R511-KMab-1] (ab264052)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [R511-KMab-1] to IDH2 (mutated R172K)
- Suitable for: Indirect ELISA, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IDH2 (mutated R172K) antibody [R511-KMab-1]
IDH2 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [R511-KMab-1] to IDH2 (mutated R172K) -
由来種
Rat -
アプリケーション
適用あり: Indirect ELISA, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
R511-KMab-1 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab264052の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Indirect ELISA |
Use at an assay dependent concentration.
Use at 312.5 ng /ml. |
|
WB |
1/1000. Predicted molecular weight: 50 kDa.
|
特記事項 |
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Indirect ELISA
Use at an assay dependent concentration. Use at 312.5 ng /ml. |
WB
1/1000. Predicted molecular weight: 50 kDa. |
ターゲット情報
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機能
Plays a role in intermediary metabolism and energy production. It may tightly associate or interact with the pyruvate dehydrogenase complex. -
関連疾患
D-2-hydroxyglutaric aciduria 2
Glioma
enetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. -
配列類似性
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
翻訳後修飾
Acetylation at Lys-413 dramatically reduces catalytic activity. Deacetylated by SIRT3. -
細胞内局在
Mitochondrion. - Information by UniProt
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参照データベース
- Entrez Gene: 3418 Human
- Omim: 147650 Human
- SwissProt: P48735 Human
- Unigene: 596461 Human
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別名
- D2HGA2 antibody
- ICD-M antibody
- IDH antibody
see all
画像
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ab264052 used at 1/1000 dilution (0.805 μg/ml).
Secondary used: Goat Anti-Rat IgG (H+L), HRP) (ab205720) at 1/10,000 dilution.
Blocking/Dilution buffer: 5% NFDM/TBST.
The loading samples are E.coil extracts containing recombinant protein respectively.
Lane 1: His-tagged human IDH1 (aa1-414) recombinant protein 10ng
Lane 2: His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng
Lane 3: His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng
Lane 4: His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng
Lane 5: His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng
Lane 6: His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng
Lane 7: His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng
Lane 8: His-tagged human IDH2 (aa40-452) recombinant protein 10ng
Lane 9: His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng
Lane 10: His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng
Lane 11: His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng
Lane 12: His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng
Lane 13: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng
Lane 14: His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ng
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ab264052 used at 0-5000 ng/ml.
Antigen (IDH2, IDH2(R172G), IDH2(R172M), IDH2(R172S), IDH2(R172W), IDH2(R172K), IDH2(R140Q)) used at 100 ng/ml.
Secondary Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at a 1/1000 dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab264052 は 2 報の論文で使用されています。
- Sabatier M et al. Activation of Vitamin D Receptor Pathway Enhances Differentiating Capacity in Acute Myeloid Leukemia with Isocitrate Dehydrogenase Mutations. Cancers (Basel) 13:N/A (2021). PubMed: 34680392
- Kaneko MK et al. Establishment of novel monoclonal antibodies KMab-1 and MMab-1 specific for IDH2 mutations. Biochem Biophys Res Commun 432:40-5 (2013). PubMed: 23376717