Anti-IDH1 (mutated R132S) 抗体 [SMab-1] (ab264049)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [SMab-1] to IDH1 (mutated R132S)
- Suitable for: Indirect ELISA, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-IDH1 (mutated R132S) antibody [SMab-1]
IDH1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [SMab-1] to IDH1 (mutated R132S) -
由来種
Mouse -
アプリケーション
適用あり: Indirect ELISA, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
SMab-1 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab264049の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Indirect ELISA |
Use a concentration of 0.949 µg/ml.
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WB |
Use a concentration of 1.186 µg/ml. Predicted molecular weight: 46 kDa.
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特記事項 |
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Indirect ELISA
Use a concentration of 0.949 µg/ml. |
WB
Use a concentration of 1.186 µg/ml. Predicted molecular weight: 46 kDa. |
ターゲット情報
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関連疾患
Glioma
Genetic variations are associated with cartilaginous tumors such as enchondroma or chondrosarcoma. Mutations of Arg-132 to Cys, Gly or His abolish the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R(-)-2-hydroxyglutarate. -
配列類似性
Belongs to the isocitrate and isopropylmalate dehydrogenases family. -
翻訳後修飾
Acetylation at Lys-374 dramatically reduces catalytic activity. -
細胞内局在
Cytoplasm. Peroxisome. - Information by UniProt
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参照データベース
- Entrez Gene: 3417 Human
- Omim: 147700 Human
- SwissProt: O75874 Human
- Unigene: 593422 Human
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別名
- Cytosolic NADP isocitrate dehydrogenase antibody
- Cytosolic NADP-isocitrate dehydrogenase antibody
- Epididymis luminal protein 216 antibody
see all
画像
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ab264049 used at 0.949µg/ml.
Goat Anti-Rat IgG (H+L), HRP) (ab205720) used as the secondary at a 1/10,000 dilution.
Blocking/Dilution buffer:5% NFDM/TBST.
Lane 1: His-tagged human IDH1 (aa1-414) recombinant protein 10ng
Lane 2: His-tagged human IDH1 mutated R132H (aa1-414) recombinant protein 10ng
Lane 3: His-tagged human IDH1 mutated R132G (aa1-414) recombinant protein 10ng
Lane 4: His-tagged human IDH1 mutated R132L (aa1-414) recombinant protein 10ng
Lane 5: His-tagged human IDH1 mutated R132S (aa1-414) recombinant protein 10ng
Lane 6: His-tagged human IDH1 mutated R132V (aa1-414) recombinant protein 10ng
Lane 7: His-tagged human IDH1 mutated R132C (aa1-414) recombinant protein 10ng
Lane 8: His-tagged human IDH2 (aa40-452) recombinant protein 10ng
Lane 9: His-tagged human IDH2 mutated R172M (aa40-452) recombinant protein 10ng
Lane 10: His-tagged human IDH2 mutated R172S (aa40-452) recombinant protein 10ng
Lane 11: His-tagged human IDH2 mutated R172G (aa40-452) recombinant protein 10ng
Lane 12: His-tagged human IDH2 mutated R172W (aa40-452) recombinant protein 10ng
Lane 13: His-tagged human IDH2 mutated R172K (aa40-452) recombinant protein 10ng
Lane 14: His-tagged human IDH2 mutated R140Q (aa40-452) recombinant protein 10ng
The loading samples are E.coil extracts containing recombinant protein respectively.
Exposure time of 3 mins. -
ELISA - Anti-IDH1 (mutated R132 S) antibody [SMab-1].
ab264049 used at 0-5000 ng/ml.
Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at a 1/1000 dilution.
Antigen concentration 1000 ng/ml.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab264049 は 1 報の論文で使用されています。
- Kaneko MK et al. Establishment of a novel monoclonal antibody SMab-1 specific for IDH1-R132S mutation. Biochem Biophys Res Commun 406:608-13 (2011). PubMed: 21352804