Recombinant human IL-7 protein (Active) (ab259382)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.050 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, MS, HPLC, Cell Culture
製品の詳細
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製品名
Recombinant human IL-7 protein (Active)
IL-7 タンパク質・ペプチド 製品一覧 -
生理活性
Fully biologically active determined by the dose dependant proliferation of 2E8 cells.
ED50 is ≤ 2 ng/mL, corresponding to a specific activity of 4.55 x 105 units/mg.
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精製度
>= 95 % SDS-PAGE.
Purity by HPLC >=95%. -
エンドトキシン・レベル
<=0.050 Eu/µg -
発現系
HEK 293 cells -
アクセッション番号
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タンパク質長
Full length protein -
Animal free
Yes -
キャリア・フリー
はい -
由来
Recombinant -
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生物種
Human -
予測される分子量
17 kDa -
分子量情報
M+1.38 Da (+365 Da: Hex(1)HexNAc(1); +203 Da: HexNAc: +283 Da: s/pGlcNac) (Calc. mass 17444.62 Da). DCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH -
領域
26 to 177 -
配列の追加情報
N-terminal glycine. Full-length mature chain lacking the signal peptide.
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特性
Our Abpromise guarantee covers the use of ab259382 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
SDS-PAGE
Functional Studies
Mass Spectrometry
HPLC
Cell Culture
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製品の状態
Lyophilized -
備考
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment
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Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at Room Temperature. Store at Room Temperature.
pH: 6.00
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose
Buffer lyophilized from.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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再構成Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
関連情報
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別名
- IL 7
- IL-7
- Il7
see all -
機能
Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. -
配列類似性
Belongs to the IL-7/IL-9 family. -
細胞内局在
Secreted. - Information by UniProt
画像
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Fully biologically active determined by the dose dependant proliferation of 2E8 cells.
ED50 is ≤ 2 ng/mL, corresponding to a specific activity of 4.55 x 105 units/mg.
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SDS-PAGE analysis of ab259382.
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Purity = 98.5%.
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 µm). 5 µL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
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M+1.38 Da (+365 Da: Hex(1)HexNAc(1); +203 Da: HexNAc: +283 Da: s/pGlcNac) (Calc. mass 17444.62 Da).
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 µm, Thermo Scientific). 5 µL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab259382 は論文での使用が確認できていません。