Anti-CD8 alpha 抗体 [BLR044F] (ab243900)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR044F] to CD8 alpha
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD8 alpha antibody [BLR044F]
CD8 alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [BLR044F] to CD8 alpha -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human CD8 alpha aa 185-235 (C terminal). The exact sequence is proprietary. NP_001759.3 and Gene ID 925.
Database link: P01732 -
ポジティブ・コントロール
- WB: CD8+ T cell whole cell lysate. IHC-P: Human tonsil and melanoma tissue.
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特記事項
This product is sold under License from Bethyl Laboratories, Inc.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading...
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特記事項(精製)
Recombinant antibody was purified from cell culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
BLR044F -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab243900の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. -
関連疾患
Defects in CD8A are a cause of familial CD8 deficiency (CD8 deficiency) [MIM:608957]. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections. -
配列類似性
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
All of the five most carboxyl-terminal cysteines form inter-chain disulfide bonds in dimers and higher multimers, while the four N-terminal cysteines do not. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 925 Human
- Omim: 186910 Human
- SwissProt: P01732 Human
- Unigene: 85258 Human
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別名
- alpha polypeptide (p32) antibody
- CD_antigen=CD8a antibody
- CD8 antibody
see all
画像
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Western blot analysis using ab243900 at 1/1000 dilution.
Lane 1: CD4+ T cell whole cell lysate (50 µg).
Lane 2: CD8+ T cell whole cell lysate (50 µg).
Lane 3: Jurkat whole cell lysate (50 µg).
Lane 4: MOLT-4 whole cell lysate (50 µg).
Lane 5: RPMI 8226 whole cell lysate (50 µg).
Lane 6: MJ whole cell lysate (50 µg).
Lane 7: C5/MJ whole cell lysate (50 µg).
Lane 8: KG-1 whole cell lysate (50 µg).
Lane 9: SR whole cell lysate (50 µg).
Lane 10: K562 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
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Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD8 alpha using ab243900 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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Formalin-fixed, paraffin-embedded human melanoma tissue stained for CD8 alpha (red) using ab243900 at 1/100 dilution in immunohistochemical analysis. A conjugated goat anti-rabbit antibody was used as the secondary. Counterstain: DAPI (blue).
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Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for CD8 alpha (red) using ab243900 at 1/250 dilution in immunohistochemical analysis. A conjugated goat anti-rabbit antibody was used as the secondary. Counterstain: DAPI (blue).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab243900 は論文での使用が確認できていません。