3D Cell Culture Clearing Kit - hydrophobic (ab243299)
製品の概要
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製品名
3D Cell Culture Clearing Kit - hydrophobic
Tissue Clearing キット 製品一覧 -
製品の概要
3D Cell Culture Clearing Kit - hydrophobic ab243299 enables the easy set-up of rapid 3D cell culture clearing for 3D imaging using simple protocols and standard laboratory equipment.
This kit can be used with immunostaining, fluorescent proteins and chemical dyes. Clearing is reversible so that you can treat your sample for conventional H&E or IHC staining after 3D imaging.
To help you get started with clearing 3D cell cultures, we have clearing validated antibodies. Validated antibodies are for key markers, such as NeuN, GFAP, Iba1, etc, and are tested for tissue clearing with 1 mm mouse brain sections, and for 3D cell culture clearing with neuronal spheroids and hepatic spheroids (depending on the marker).
If you want to set up clearing of 3D cell cultures on a sample type that we haven’t tested or to use your own antibodies, please consult the protocol book for this kit.
To learn more about tissue clearing and clearing of 3D cell cultures, please review our guide to tissue clearing and clearing 3D cell cultures.
Clearing reagents and companion products are also available as individual products.
These clearing reagents were developed with Visikol Inc, who developed the Visikol® HISTO™ clearing technology which is the basis of our reagents.
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特記事項
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
アプリケーション
適用あり: Histologymore details
製品の特性
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保存方法
Please refer to protocols. -
内容 30 ml 15 ml 3D Cell Culture Clearing Reagent 1 x 30ml 1 x 15ml Tissue Clearing Antibody Buffer 1 x 30ml 1 x 15ml Tissue Clearing Blocking Buffer 1 x 30ml 1 x 15ml Tissue Clearing Penetration Buffer 1 x 30ml 1 x 15ml Tissue Clearing Washing Buffer 10X 1 x 70ml 1 x 35ml -
関連性
The goal for tissue clearing is making large, fixed biological samples transparent. “Large” in this case means thick sections of tissue, whole organoids, entire organs or even entire young rats, ranging in thickness from around 100 µm to several centimeters. Typically, samples of this size are not transparent, and are therefore difficult to analyze using visible wavelengths of light with a microscope. Tissue clearing involves a series of chemical steps that render a large sample transparent.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab243299の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Histology |
Use at an assay dependent concentration.
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特記事項 |
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Histology
Use at an assay dependent concentration. |
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab243299 は論文での使用が確認できていません。