Anti-MD2 抗体 (ab24182)
Key features and details
- Rabbit polyclonal to MD2
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-MD2 antibody
MD2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MD2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
精製度
Affinity purified -
特記事項(精製)
Via peptide column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Positive Controls
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24182の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | (5) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 18 kDa).
|
ICC/IF | (2) |
Use at an assay dependent concentration.
|
IHC-P | (5) |
Use at an assay dependent concentration.
|
特記事項 |
---|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 18 kDa). |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both MD2 and TLR4, but not TLR4 alone, respond to LPS. -
翻訳後修飾
N-glycosylated; high-mannose. -
細胞内局在
Secreted > extracellular space. - Information by UniProt
-
参照データベース
- Entrez Gene: 23643 Human
- Entrez Gene: 17087 Mouse
- Entrez Gene: 448830 Rat
- Omim: 605243 Human
- SwissProt: Q9Y6Y9 Human
- SwissProt: Q9JHF9 Mouse
- Unigene: 726603 Human
- Unigene: 116844 Mouse
-
別名
- ESOP 1 antibody
- ESOP-1 antibody
- ESOP1 antibody
see all
画像
-
All lanes : Anti-MD2 antibody (ab24182) at 2 µg/ml (in 5% milk in TBS/T 0.1% (blocking buffer) for 12 hours at 4°C)
Lane 1 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours
Lane 2 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and LPS (50 ng/ml) for 10 minutes
Lane 3 : Whole cell lysate of rat cortical astrocytes treated with control siRNA for 96 hours and ethanol (50 mM) for 10 minutes
Lane 4 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours
Lane 5 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and LPS (50 ng/ml) for 10 minutes
Lane 6 : Whole cell lysate of rat cortical astrocytes treated with MD2 siRNA for 96 hours and ethanol (50 mM) for 10 minutes
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : An HRP-conjugated Goat anti-rabbit IgG at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 18 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes
Blocking Step: 5% Milk for 1 hour at room temperature. -
Anti-MD2 antibody (ab24182) at 1 µg/ml + Daudi cell lysate at 15 µg
Secondary
Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 18 kDa -
All lanes : Anti-MD2 antibody (ab24182) at 1 µg/ml
Lane 1 : Mouse spleen lysate
Lane 2 : Mouse thymus lysate
Lane 3 : Mouse testis lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 18 kDa -
All lanes : Anti-MD2 antibody (ab24182)
All lanes : Rat spleen lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 18 kDa -
Anti-MD2 antibody (ab24182) at 1 µg/ml (mouse spleen lysate)
Predicted band size: 18 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted? -
ab24182 at 2µg/ml staining of MD2 in rat spleen cells by IHC
-
-
-
Immunohistochemical analysis of human spleen tissue stained for MD2 using ab24182 at 2.5μg/mL.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (38)
ab24182 は 38 報の論文で使用されています。
- Jiang H et al. Innate/Inflammatory Bioregulation of Surfactant Protein D Alleviates Rat Osteoarthritis by Inhibiting Toll-Like Receptor 4 Signaling. Front Immunol 13:913901 (2022). PubMed: 35865531
- Shi L et al. HDAC6 Inhibition Alleviates Ischemia- and Cisplatin-Induced Acute Kidney Injury by Promoting Autophagy. Cells 11:N/A (2022). PubMed: 36552715
- Huang S et al. Myeloid differentiation 2 deficiency attenuates AngII-induced arterial vascular oxidative stress, inflammation, and remodeling. Aging (Albany NY) 13:4409-4427 (2021). PubMed: 33495414
- Zhang P et al. Soluble MD-2 and Heme in Sickle Cell Disease Plasma Promote Pro-Inflammatory Signaling in Endothelial Cells. Front Immunol 12:632709 (2021). PubMed: 33841413
- Zhang T et al. Artemisinin inhibits TLR4 signaling by targeting co-receptor MD2 in microglial BV-2 cells and prevents lipopolysaccharide-induced blood-brain barrier leakage in mice. J Neurochem 157:611-623 (2021). PubMed: 33453127