Anti-USP16 抗体 [EPR20914-47] (ab236628)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20914-47] to USP16
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-USP16 antibody [EPR20914-47]
USP16 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR20914-47] to USP16 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP -
種交差性
交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Rat testis and liver lysates; Mouse testis lysate; MEF whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR20914-47 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236628の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 94 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 94 kDa). |
ターゲット情報
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機能
Specifically deubiquitinates histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A is a prerequisite for subsequent phosphorylation at 'Ser-10' of histone H3, and is required for chromosome segregation when cells enter into mitosis. Regulates Hox gene expression via histone H2A deubiquitination. Prefers nucleosomal substrates. Does not deubiquitinate histone H2B. -
組織特異性
Present in all the tissues examined including fetal brain, lung, liver, kidney, and adult heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. -
関連疾患
Note=A chromosomal aberration involving USP16 is a cause of Chronic myelomonocytic leukemia. Inversion inv(21) (q21;q22) with RUNX1/AML1. -
配列類似性
Belongs to the peptidase C19 family. USP16 subfamily.
Contains 1 UBP-type zinc finger. -
ドメイン
The UBP-type zinc finger binds 3 zinc ions that form a pair of cross-braced ring fingers encapsulated within a third zinc finger in the primary structure. It recognizes the C-terminal tail of free ubiquitin. -
翻訳後修飾
Phosphorylated at the onset of mitosis and dephosphorylated during the metaphase/anaphase transition. The phosphorylated form of the protein is also enzymatically active. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 74112 Mouse
- Entrez Gene: 288306 Rat
- GenBank: NM_024258 Mouse
- SwissProt: Q99LG0 Mouse
- SwissProt: Q2KJ09 Rat
- Unigene: 152941 Mouse
- Unigene: 55800 Rat
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別名
- Deubiquitinating enzyme 16 antibody
- Human ubiquitin processing protease antibody
- MSTP039 antibody
see all
画像
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All lanes : Anti-USP16 antibody [EPR20914-47] (ab236628) at 1/1000 dilution
Lane 1 : Rat testis tissue lysate at 20 µg
Lane 2 : Rat liver tissue lysate at 20 µg
Lane 3 : Mouse testis tissue lysate at 20 µg
Lane 4 : MEF (mouse embryonic fibroblast (immortalized)), whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 94 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 26323689)
Exposure time: Lanes 1-3: 3 minutes Lane 4: 48 seconds
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab236628 は論文での使用が確認できていません。