Anti-ICOS Ligand/ICOSL 抗体 [EPR23289-152] (ab233554)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23289-152] to ICOS Ligand/ICOSL
- Suitable for: Flow Cyt
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ICOS Ligand/ICOSL antibody [EPR23289-152]
ICOS Ligand/ICOSL 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23289-152] to ICOS Ligand/ICOSL -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cytmore details
適用なし: ICC/IF,IHC-P,IP or WB -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow Cyt: Jurkat, HUVEC cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23289-152 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab233554の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
1/50.
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特記事項 |
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Flow Cyt
1/50. |
ターゲット情報
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機能
Ligand for the T-cell-specific cell surface receptor ICOS. Acts as a costimulatory signal for T-cell proliferation and cytokine secretion; induces also B-cell proliferation and differentiation into plasma cells. Could play an important role in mediating local tissue responses to inflammatory conditions, as well as in modulating the secondary immune response by co-stimulating memory T-cell function. -
組織特異性
Isoform 1 is widely expressed (brain, heart, kidney, liver, lung, pancreas, placenta, skeletal muscle, bone marrow, colon, ovary, prostate, testis, lymph nodes, leukocytes, spleen, thymus and tonsil), while isoform 2 is detected only in lymph nodes, leukocytes and spleen. Expressed on activated monocytes and dendritic cells. -
配列類似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 23308 Human
- Omim: 605717 Human
- SwissProt: O75144 Human
- Unigene: 14155 Human
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別名
- B7 H2 antibody
- B7 homolog 2 antibody
- B7 homologue 2 antibody
see all
画像
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Flow cytometric analysis of HUVEC (Human umbilical vein endothelial cell) cells treated with 200U/ml TNF alpha for 24h / Untreated control (Green) labelling ICOS Ligand/ICOSL with ab233554 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells (PMID: 16210590).
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Flow cytometric analysis of Jurkat (Human T cell leukemia T lymphocyte, Left) / Raji(Human Burkitt's lymphoma B lymphocyte, Right) cells labelling ICOS Ligand/ICOSL with ab233554 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Negative control: Jurkat (PMID: 21851236). Gated on viable cells.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab233554 は論文での使用が確認できていません。