Anti-GSDMC2 + GSDMC3 抗体 [EPR20890-48] (ab229896)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20890-48] to GSDMC2 + GSDMC3
- Suitable for: IHC-P, IHC-Fr, WB
- Knockout validated
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48]
GSDMC2+GSDMC3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR20890-48] to GSDMC2 + GSDMC3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IHC-Fr, WBmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Mouse -
免疫原
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. -
ポジティブ・コントロール
- WB: Mouse stomach, small intestine, colon and caecum tissue lysates; HEK-293T transfected with mGSDMC2 expression vector whole cell lysate; HEK-293T transfected with mGSDMC3 expression vector whole cell lysate. IHC-P: Mouse stomach tissue. IHC-Fr: Mouse stomach and colon tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR20890-48 -
アイソタイプ
IgG
関連製品
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Alternative Versions
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Compatible Secondaries
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab229896の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
1/200.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
WB |
1/1000. Detects a band of approximately 54, 56 kDa (predicted molecular weight: 54 kDa).
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特記事項 |
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IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/200. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
WB
1/1000. Detects a band of approximately 54, 56 kDa (predicted molecular weight: 54 kDa). |
ターゲット情報
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細胞内局在
GSDMC2: Cytoplasm, cytosol. Cell membrane. -
参照データベース
- Entrez Gene: 270328 Mouse
- Entrez Gene: 331063 Mouse
- SwissProt: Q2KHK6 Mouse
- SwissProt: Q8CB12 Mouse
- Unigene: 273185 Mouse
- Unigene: 483471 Mouse
画像
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Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling GSDMC2+GSDMC3 with abab229896 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplamic staining on epithelial cells in mouse stomach (PMID: 17350798). The section was incubated with ab229896 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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All lanes : Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896) at 1/1000 dilution
Lane 1 : Wild-type mouse esophagus tissue lysate
Lane 2 : mGSDMC1-4 knockout mouse esophagus tissue lysate
Lane 3 : Wild-type mouse stomach tissue lysate
Lane 4 : mGSDMC1-4 knockout mouse stomach tissue lysate
Lane 5 : Wild-type mouse small intestine tissue lysate
Lane 6 : mGSDMC1-4 knockout mouse small intestine tissue lysate
Lane 7 : Wild-type mouse colon tissue lysate
Lane 8 : mGSDMC1-4 knockout mouse colon tissue lysate
Lane 9 : Wild-type mouse caecum tissue lysate
Lane 10 : mGSDMC1-4 knockout mouse caecum tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).
Exposure time: 60 seconds.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse colon tissue labeling GSDMC2+GSDMC3 with ab229896 at 1/200 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on epithelial cells in mouse colon is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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All lanes : Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896) at 1/1000 dilution
Lane 1 : Mouse stomach tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Negative control: spleen (PMID: 17350798).
Exposure time: 54 seconds.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling GSDMC2+GSDMC3 with ab229896 at 1/200 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on epithelial cells in mouse stomach is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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All lanes : Anti-GSDMC2 + GSDMC3 antibody [EPR20890-48] (ab229896) at 1/1000 dilution
Lane 1 : HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), whole cell lysate
Lane 2 : HEK-293T transfected with mGSDMC1 expression vector, whole cell lysate
Lane 3 : HEK-293T transfected with mGSDMC2 expression vector, whole cell lysate
Lane 4 : HEK-293T transfected with mGSDMC3 expression vector, whole cell lysate
Lane 5 : HEK-293T transfected with mGSDMC4 expression vector, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The image was kindly provided by Dr. Jiahuai Han (Xiamen University, China).
Exposure time: 60 seconds.
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GSDMC2+GSDMC3 with abab229896 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: No staining on mouse spleen (PMID: 17350798). The section was incubated with ab229896 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab229896 は 2 報の論文で使用されています。
- Zhao M et al. Epithelial STAT6 O-GlcNAcylation drives a concerted anti-helminth alarmin response dependent on tuft cell hyperplasia and Gasdermin C. Immunity 55:623-638.e5 (2022). PubMed: 35385697
- Xi R et al. Up-regulation of gasdermin C in mouse small intestine is associated with lytic cell death in enterocytes in worm-induced type 2 immunity. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34290141