Anti-Chromogranin A 抗体 [CHGA/777] (ab220190)
Key features and details
- Mouse monoclonal [CHGA/777] to Chromogranin A
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Chromogranin A antibody [CHGA/777]
Chromogranin A 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [CHGA/777] to Chromogranin A -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
非交差種: Rat -
免疫原
Recombinant full length protein corresponding to Human Chromogranin A.
Database link: P10645 -
ポジティブ・コントロール
- Human Panc-1 cell lysate;Human pheochromocytoma, pancreas and adrenal gland tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
ab220190 is purified from Bioreactor Concentrate by Protein A/G column chromatography. -
ポリ/モノ
モノクローナル -
クローン名
CHGA/777 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab220190の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For 30 minutes at RT. |
|
WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.
|
特記事項 |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For 30 minutes at RT. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa. |
ターゲット情報
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機能
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. -
配列類似性
Belongs to the chromogranin/secretogranin protein family. -
翻訳後修飾
Sulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
細胞内局在
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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参照データベース
- Entrez Gene: 1113 Human
- Omim: 118910 Human
- SwissProt: P10645 Human
- Unigene: 150793 Human
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製品の状態
According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa. -
別名
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
see all
画像
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pheochromocytoma tissue labeling Chromogranin A with ab220190 at 1 µg/ml.
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Anti-Chromogranin A antibody [CHGA/777] (ab220190) at 1 µg/ml + Panc-1 cell lysate
Predicted band size: 50 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Chromogranin A with ab220190 at 1 µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adrenal gland tissue labeling Chromogranin A with ab220190 at 1 µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab220190 は論文での使用が確認できていません。