Anti-Fibrillarin 抗体 [4A4] (ab218846)
Key features and details
- Mouse monoclonal [4A4] to Fibrillarin
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Fibrillarin antibody [4A4]
Fibrillarin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [4A4] to Fibrillarin -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant full length protein corresponding to Human Fibrillarin aa 1 to the C-terminus. (Purified from E.coli).
Database link: P22087 -
ポジティブ・コントロール
- WB: NIH/3T3, HEK-293, HeLa and SH-SY5Y cell lysates. ICC/IF: HEK-293, C6 and HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.03% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
4A4 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab218846の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/5000. Predicted molecular weight: 33 kDa.
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ICC/IF |
1/1000 - 1/2000.
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特記事項 |
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WB
1/2000 - 1/5000. Predicted molecular weight: 33 kDa. |
ICC/IF
1/1000 - 1/2000. |
ターゲット情報
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機能
S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239). -
配列類似性
Belongs to the methyltransferase superfamily. Fibrillarin family. -
翻訳後修飾
By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA). -
細胞内局在
Nucleus, nucleolus. Fibrillar region of the nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 2091 Human
- Entrez Gene: 102643269 Mouse
- Entrez Gene: 14113 Mouse
- Entrez Gene: 292747 Rat
- Omim: 134795 Human
- SwissProt: P22087 Human
- SwissProt: P35550 Mouse
- SwissProt: P22509 Rat
see all -
別名
- 34 kD nucleolar scleroderma antigen antibody
- 34 kDa nucleolar scleroderma antigen antibody
- Fbl antibody
see all
画像
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C6 (rat glial tumor cell line) (Panel A) and HEK-293 (human epithelial cell line from embryonic kidney) (Panel B) cells stained for Fibrillarin (red) using ab218846 at 1/1000 dilution in ICC/IF. Co-stained with chicken pAb to vimentin, at 1/10000 dilution (green). The blue is DAPI staining of nuclear DNA.
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All lanes : Anti-Fibrillarin antibody [4A4] (ab218846) at 1/2000 dilution
Lane 1 : MW ladder
Lane 2 : Rat whole brain lysate
Lane 3 : Mouse whole brain lysate
Lane 4 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lane 5 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lane 6 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 7 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate
Predicted band size: 33 kDaFibrillarin detection is shown in green.
Also stained with a rabbit antibody to UCHL-1 at 1/3000 dilution (red).
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Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Fibrillarin with ab218846 at 1/1000 dilution (red). The cells were counterstained with a chicken antibody to vimentin at 1/5000 dilution (green) and DAPI to label the nuclei (blue).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab218846 は 1 報の論文で使用されています。
- Velásquez ZD et al. Eimeria bovis infections induce G1 cell cycle arrest and a senescence-like phenotype in endothelial host cells. Parasitology 148:341-353 (2021). PubMed: 33100232